Jyonouchi H, Voss R M, Good R A
Department of Pediatrics/St. Petersburg, University of South Florida College of Medicine/All Children's Hospital 33701.
Proc Soc Exp Biol Med. 1991 Dec;198(3):818-25. doi: 10.3181/00379727-198-43319.
CD5 expression on B cells is regulated by certain humoral factors. In a pre-B leukemia cell line 70Z/3, we found that interleukin 4 down-regulates it. Herein, we report that zinc influences spontaneous CD5 expression by this cell line as well as actions of these factors on CD5 expression considerably. In zinc-depleted culture media, spontaneous CD5 expression by 70Z/3 cells was enhanced. In contrast, the down-regulatory action of interleukin 4 was significantly reduced under culture conditions of zinc depletion. The supplementation of zinc to physiologic concentrations (1 to 2 microM) abolished such effects of zinc-depleted medium. The reduction of the suppressive action of interleukin 4 was observed at the level of gene expression. However, CD5 mRNA expression enhanced by lipopolysaccharide or NZB-SF was not further enhanced under conditions of zinc deficiency. These observations may suggest that CD5 expression by malignant or even normal B cells may be influenced by cellular/serum zinc levels.
B细胞上CD5的表达受某些体液因子调控。在一个前B白血病细胞系70Z/3中，我们发现白细胞介素4可下调其表达。在此，我们报告锌可影响该细胞系的自发CD5表达，并且这些因子对CD5表达的作用也会受到显著影响。在缺锌的培养基中，70Z/3细胞的自发CD5表达增强。相反，在缺锌的培养条件下，白细胞介素4的下调作用显著降低。将锌补充至生理浓度（1至2微摩尔）可消除缺锌培养基的此类影响。白细胞介素4抑制作用的降低在基因表达水平即可观察到。然而，在缺锌条件下，脂多糖或NZB-SF增强的CD5 mRNA表达并未进一步增强。这些观察结果可能表明，恶性甚至正常B细胞的CD5表达可能受细胞/血清锌水平的影响。
