Raveche E S, Lin T Z, Conroy J, Tjio J H, Steinberg A D, Rudolfsky U A, Phillips J A
Dept. of Pathology, UMDNJ, New Jersey Medical School, Newark 07103.
Autoimmunity. 1991;10(3):217-25. doi: 10.3109/08916939109001892.
Autoimmune NZB mice have increased percentages of CD5+B (Lyl+B) cells in both the spleen and peritoneum. We have previously reported that as NZB mice age they develop a clonal population of hyperdiploid CD5+B cells in the spleen. These cells can readily be transplanted into unirradiated recipients. The growth characteristics of such transplanted hyperdiploid NZB spleen cells were examined in different recipient strains to determine if the immunological status of the host environments affected the growth of the clonal CD5+B cells. Young NZB and NZB.xid recipients (lacking hyperdiploid CD5+B cells) allowed growth and expansion of unpassaged CD5+B cells derived from primary NZB mice. Similarly, (NZBxDBA/2) and (NZBxBALB/c) F1 recipients allowed for expansion of CD5+B cell clones from primary sources. In a separate experiment, T cell-depleted NZB spleen cells containing a hyperdiploid CD5+B cell clone were transferred to SCID mice. The SCID environment supported the growth of the primary clone. None of these recipients normally have elevated CD5+B cells, yet these recipients allowed growth of primary transferred hyperdiploid cells. However, a difference in the ability of these recipient strains in their ability to expand multiply passaged CD5+B cell clones was observed. These results indicate that while hyperdiploid CD5+B cells are difficult to be maintained in culture, they can readily be passaged in vivo. The host environment may provide growth factors or signals for endogenous growth factors. Although the CD5+B clones arise initially in a hyperactive autoimmune environment, a hyperimmune environment is not necessary to support their growth. Transferred CD5+B cells affect the recipient environment and reduce the percentages of normal B cells.
自身免疫性新西兰黑鼠（NZB）的脾脏和腹膜中CD5⁺B（Lyl⁺B）细胞的百分比增加。我们之前报道过，随着NZB小鼠年龄增长，它们的脾脏中会出现一群超二倍体CD5⁺B细胞的克隆群体。这些细胞可以很容易地移植到未受辐照的受体中。在不同的受体品系中检测了这种移植的超二倍体NZB脾细胞的生长特性，以确定宿主环境的免疫状态是否会影响克隆性CD5⁺B细胞的生长。年轻的NZB和NZB.xid受体（缺乏超二倍体CD5⁺B细胞）允许来自初代NZB小鼠的未传代CD5⁺B细胞生长和扩增。同样，（NZB×DBA/2）和（NZB×BALB/c）F1受体允许来自原始来源的CD5⁺B细胞克隆扩增。在另一个实验中，将含有超二倍体CD5⁺B细胞克隆的T细胞耗竭的NZB脾细胞转移到SCID小鼠中。SCID环境支持原始克隆的生长。这些受体通常都没有升高的CD5⁺B细胞，但这些受体允许原始转移的超二倍体细胞生长。然而，观察到这些受体品系在扩增多次传代的CD5⁺B细胞克隆的能力上存在差异。这些结果表明，虽然超二倍体CD5⁺B细胞在培养中难以维持，但它们可以很容易地在体内传代。宿主环境可能提供生长因子或内源性生长因子的信号。虽然CD5⁺B克隆最初出现在高度活跃的自身免疫环境中，但超免疫环境并非支持其生长所必需的。转移的CD5⁺B细胞会影响受体环境并降低正常B细胞的百分比。
