使用傅里叶域、角分辨低相干干涉测量法进行快速、深度分辨光散射测量。
Rapid, depth-resolved light scattering measurements using Fourier domain, angle-resolved low coherence interferometry.
作者信息
Pyhtila John W, Wax Adam
机构信息
Department of Biomedical Engineering and the Fitzpatrick Center for Photonics and Communication Systems, Duke University, Durham, NC 27708
出版信息
Opt Express. 2004 Dec 13;12(25):6178-83. doi: 10.1364/opex.12.006178.
We present a novel angle-resolved low coherence interferometry scheme for rapid measurement of depth-resolved angular scattering distributions to enable determination of scatterer size via elastic scattering properties. Depth resolution is achieved using a superluminescent diode in a modified Mach-Zehnder interferometer with the mixed signal and reference fields dispersed by an imaging spectrograph. The spectrograph slit is located in a Fourier transform plane of the scattering sample, enabling angle-resolved measurements over a 0.21 radian range. The capabilities of the new technique are demonstrated by recording the distribution of light scattered by a sub-surface layer of polystyrene microspheres in 40 milliseconds. The data are used to determine the microsphere size with good accuracy. Future clinical application to measuring the size of cell nuclei in living epithelial tissues using backscattered light is discussed.
我们提出了一种新颖的角分辨低相干干涉测量方案，用于快速测量深度分辨的角散射分布，以便通过弹性散射特性确定散射体大小。在改进的马赫曾德尔干涉仪中使用超发光二极管实现深度分辨率，混合信号和参考场由成像光谱仪色散。光谱仪狭缝位于散射样品的傅里叶变换平面中，能够在0.21弧度范围内进行角分辨测量。通过在40毫秒内记录聚苯乙烯微球亚表层散射光的分布，展示了这项新技术的能力。这些数据用于高精度地确定微球大小。还讨论了该技术未来在利用背散射光测量活体上皮组织中细胞核大小的临床应用。
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