Baicu Simona C, Taylor Michael J, Brockbank Kelvin G M
Cell and Tissue Systems, Inc., 2231 Technical Parkway, Suite A, N. Charleston, SC 29406, USA.
Cryobiology. 2007 Feb;54(1):114-20. doi: 10.1016/j.cryobiol.2006.11.001. Epub 2006 Dec 28.
This study documents renal biochemistry during hypothermic machine perfusion of kidneys. It is intended to demonstrate that a comprehensive evaluation of organ viability during ex-vivo preservation is needed to increase the number of organs available for transplantation and to reduce the current renal discard rate. Porcine kidneys were hypothermically machine perfused for 72 h with either Unisol-UHK or Belzer-Machine Perfusion Solution, (Belzer-MPS). Renal perfusate samples were periodically collected and biochemically analyzed. Significant differences were measured in the renal metabolic activity between the two experimental groups while similar values for traditional parameters such as renal flow rate and vascular resistance values were recorded. The effluent of UHK perfused kidneys showed strong metabolites and NH(4)(+) dynamics (P<0.05 vs. baseline), while the Belzer-MPS kidneys metabolic activity led to little or no change of the effluent biochemistry relative to baseline.
本研究记录了肾脏低温机器灌注过程中的肾脏生物化学情况。旨在证明，在体外保存期间需要对器官活力进行全面评估，以增加可用于移植的器官数量，并降低当前的肾脏丢弃率。用Unisol-UHK或Belzer机器灌注液（Belzer-MPS）对猪肾进行72小时的低温机器灌注。定期收集肾脏灌注液样本并进行生化分析。在两个实验组之间测量到肾脏代谢活性存在显著差异，而记录的传统参数（如肾血流量和血管阻力值）相似。UHK灌注肾脏的流出液显示出强烈的代谢物和NH(4)(+)动态变化（与基线相比，P<0.05），而Belzer-MPS灌注肾脏的代谢活性导致流出液生化指标相对于基线几乎没有变化或没有变化。
