Shao Hong-Bo, Chu Li-Ye, Wu Gang, Zhang Jin-Heng, Lu Zhao-Hua, Hu Ya-Chen
Binzhou University, Binzhou 256603, China.
Colloids Surf B Biointerfaces. 2007 Feb 15;54(2):143-9. doi: 10.1016/j.colsurfb.2006.09.004. Epub 2006 Sep 12.
Drought is one of the major ecological factors limiting crop production and food quality globally, especially in the arid and semi-arid areas of the world. Wheat is the staple food for more than 35% of world population and wheat cultivation is mainly restricted to such zones with scarcity of water, so wheat anti-drought physiology study is of importance to wheat production, food safety and quality and biotechnological breeding for the sake of coping with abiotic and biotic conditions. The current study is to investigate changes of anti-oxidative physiological indices of 10 wheat genotypes at tillering stage. The main results and conclusion of tillering stage in terms of activities of POD, SOD, CAT and MDA content as followed: (1) 10 wheat genotypes can be generally grouped into three kinds (A-C, respectively) according to their changing trend of the measured indices; (2) A group performed better drought resistance under the condition of treatment level 1 (appropriate level), whose activities of anti-oxidative enzymes (POD, SOD, CAT) were higher and MDA lower; (3) B group exhibited stronger anti-drought under treatment level 2 (light-stress level), whose activities of anti-oxidative enzymes were higher and MDA lower; (4) C group expressed anti-drought to some extent under treatment level 3 (serious-stress), whose activities of anti-oxidative enzymes were stronger, MDA lower; (5) these results demonstrated that different wheat genotypes have different physiological mechanisms to adapt themselves to changing drought stress, whose molecular basis is discrete gene expression profiling (transcriptom). The study in this respect is the key to wheat anti-drought and biological-saving water in worldwide arid and semi-arid areas; (6) POD, SOD, and CAT activities and MDA content of different wheat genotypes had quite different changing trend at different stages and under different soil water stress conditions, which was linked with their origin of cultivation and individual soil water threshold, which will provide better reference to selecting proper plant species for eco-environmental construction and crops for sustainable agriculture in arid and semi-arid areas.
干旱是全球范围内限制作物产量和食品质量的主要生态因素之一，尤其是在世界干旱和半干旱地区。小麦是超过35%世界人口的主食，而小麦种植主要局限于水资源稀缺的地区，因此小麦抗旱生理学研究对于小麦生产、食品安全与质量以及生物技术育种以应对非生物和生物条件而言至关重要。当前研究旨在调查10个小麦基因型在分蘖期抗氧化生理指标的变化。分蘖期POD、SOD、CAT活性及MDA含量方面的主要结果与结论如下：（1）根据所测指标的变化趋势，10个小麦基因型总体可分为三类（分别为A - C）；（2）A组在处理水平1（适宜水平）条件下表现出较好的抗旱性，其抗氧化酶（POD、SOD、CAT）活性较高而MDA较低；（3）B组在处理水平2（轻度胁迫水平）下表现出较强的抗旱性，其抗氧化酶活性较高而MDA较低；（4）C组在处理水平3（重度胁迫）下表现出一定程度的抗旱性，其抗氧化酶活性较强，MDA较低；（5）这些结果表明不同小麦基因型具有不同的生理机制以适应不断变化的干旱胁迫，其分子基础是离散的基因表达谱（转录组）。这方面的研究是全球干旱和半干旱地区小麦抗旱及生物节水的关键；（6）不同小麦基因型的POD、SOD、CAT活性及MDA含量在不同阶段和不同土壤水分胁迫条件下具有截然不同的变化趋势，这与它们的种植起源和个体土壤水分阈值有关，这将为干旱和半干旱地区生态环境建设选择合适植物物种以及可持续农业选择作物提供更好的参考。
