Yaadesh Sivakumar, Tomar Govind Singh, Kaushik Rajeev, Prasanna Radha, Grover Minakshi
Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.
3 Biotech. 2023 Mar;13(3):90. doi: 10.1007/s13205-023-03503-4. Epub 2023 Feb 20.
The association of plant beneficial and spp. strains expressing different sets of PGP traits may have complementary or supplementary effects on host plants. In the present investigation, and spp. strains showing diverse PGP traits (IAA production, nitrogenase activity, phosphate, zinc and potassium solubilization, siderophores, antagonism against phytopathogens, osmotic stress tolerance, etc.) were assessed for compatibility by cross-streaking and co-culturing. Under co-culture ( + ), a significant increase in the expression of PGP traits, nitrogenase activity (up to 89%), phosphate solubilization (upto 236%), siderophore production (upto 20%) was observed as compared to individual culture, indicating synergistic effect of co-culture. IAA production was higher in sp. strains as compared to spp. strains, when cultured individually; however, when co-cultured, the IAA levels were in the mid-range indicating the contributory effects of compatible strains. The effect of individual and strains and their co-inoculation was also assessed on the growth of pearl millet at early stages under moisture-deficit stress imposed using PEG6000 (0, 10, and 20%). Co-inoculation enhanced seed germination (up to 10, 3, and 6% increase under 0, 10, and 20% PEG, respectively, over individual treatment), root traits (increased root hair density and lateral branches), and seedling vigor indices (up to 22, 32, 43% increase in seed vigor index I and 8, 14, and 10% increase in seed vigor index II under 0, 10, 20% PEG, respectively, over individual treatment) under normal as well as moisture-deficit conditions suggesting the role of spp. strains in better adaptation of the plants to stress and higher yield potential. The synergistic effect of co-cultured and strains on PGP traits indicated metabolic interplay between the two strains which needs to be further understood. The positive effect of co-inoculation on plant growth under moisture-deficit stress indicated the promise of and as a synergistic bioformulation for combating nutrient and drought stress in pearl millet, particularly in nutrient-poor dryland agricultural systems.
The online version contains supplementary material available at 10.1007/s13205-023-03503-4.
表达不同植物生长促进（PGP）性状组合的植物有益菌和 spp. 菌株对宿主植物可能具有互补或补充作用。在本研究中，通过交叉划线和共培养评估了表现出不同PGP性状（吲哚-3-乙酸（IAA）产生、固氮酶活性、磷酸盐、锌和钾溶解、铁载体、对植物病原体的拮抗作用、渗透胁迫耐受性等）的 和 spp. 菌株的兼容性。在共培养（ + ）条件下，与单独培养相比，观察到PGP性状的表达、固氮酶活性（高达89%）、磷酸盐溶解（高达236%）、铁载体产生（高达20%）显著增加，表明共培养具有协同效应。单独培养时， sp. 菌株中的IAA产量高于 spp. 菌株；然而，共培养时，IAA水平处于中等范围，表明兼容菌株的促进作用。还评估了单独的 和 菌株及其共同接种对在使用聚乙二醇6000（PEG6000，0、10和20%）施加水分亏缺胁迫下珍珠粟早期生长的影响。在正常和水分亏缺条件下，共同接种均提高了种子发芽率（在0、10和20%PEG条件下，分别比单独接种 处理提高10%、3%和6%）、根系性状（增加根毛密度和侧枝）以及幼苗活力指数（在0、10、20%PEG条件下，种子活力指数I分别比单独接种 处理提高22%、32%、43%，种子活力指数II分别提高8%、14%、10%），表明 spp. 菌株在植物更好地适应胁迫和提高产量潜力方面的作用。共培养的 和 菌株对PGP性状的协同效应表明这两种菌株之间存在代谢相互作用，这需要进一步了解。在水分亏缺胁迫下共同接种对植物生长的积极影响表明， 和 作为一种协同生物制剂有望用于应对珍珠粟的养分和干旱胁迫，特别是在养分贫瘠的旱地农业系统中。
在线版本包含可在10.1007/s13205-023-03503-4获取的补充材料。
