Stefanova Tsvetanka, Nikolova Nadja, Michailova Anastasija, Mitov Ivan, Iancov Ianco, Zlabinger Gerhard J, Neychev Hristo
Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 26 Acad. G. Bonchev St., Sofia 1113, Bulgaria.
Microbes Infect. 2007 Jan;9(1):7-14. doi: 10.1016/j.micinf.2006.10.001. Epub 2006 Dec 8.
Coumarin and its derivatives are naturally occurring substances with multiple biological activities. Here we demonstrate that prophylactic peroral administration of coumarin or 7-hydroxycoumarin (7-OHC) enhances resistance to subsequent lethal Salmonella enterica Serovar Typhimurium infection in mice. 7-OHC decreased bacterial load in liver and spleen, and enhanced phagocytosis and bacterial killing by macrophages when applied in vitro and in vivo. 7-OHC treatment induced significant NO release in peritoneal macrophage cultures. The observed protective effect correlated with the induction of Th1-associated cytokines, such as IL-12, IFN-gamma, and TNF-alpha. These data demonstrate a clear immunomodulatory potential of coumarins which might have important therapeutic implications to enhance resistance to infection.
香豆素及其衍生物是具有多种生物活性的天然存在物质。在此我们证明，预防性经口给予香豆素或7-羟基香豆素（7-OHC）可增强小鼠对随后致死性肠炎沙门氏菌鼠伤寒血清型感染的抵抗力。7-OHC降低了肝脏和脾脏中的细菌载量，并在体外和体内应用时增强了巨噬细胞的吞噬作用和细菌杀伤能力。7-OHC处理在腹膜巨噬细胞培养物中诱导了显著的一氧化氮释放。观察到的保护作用与Th1相关细胞因子如IL-12、IFN-γ和TNF-α的诱导相关。这些数据证明香豆素具有明确的免疫调节潜力，这可能对增强抗感染能力具有重要的治疗意义。
