Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Immunol Invest. 2012;41(2):199-213. doi: 10.3109/08820139.2011.619021. Epub 2011 Oct 18.
Coumarin and its derivatives have potent immunomodulatory activities. Here we describe the parameters of the protective effect of 7-hydroxycoumarin (7-OHC) in experimental Salmonella enterica Serovar Typhimurium infection in mice. The protective effect depended on the duration of treatment reaching its maximum after 10 days of pretreatment and lasted for at least 15 days after its end. Electron microscopy studies revealed that 7-OHC induced ultrastructural changes in macrophages consistent with their activation as well as faster destruction of ingested salmonellae. Superoxide and hydrogen peroxide secretion by macrophages was decreased in both healthy and Salmonella-infected 7-OHC treated animals, which is in line with the current view that some coumarins possess antioxidant and radical scavenging activity. Thus, 7-OHC pretreatment also appears beneficial to the host by limiting the harmful tissue damaging and immunosuppressive effects of the oxidative stress during a Salmonella infection but still activates the microbicidal capacity of exposed phagocytes.
香豆素及其衍生物具有很强的免疫调节活性。在这里，我们描述了 7-羟基香豆素（7-OHC）在实验性鼠伤寒沙门氏菌感染中的保护作用的参数。保护作用取决于治疗的持续时间，在预处理 10 天后达到最大值，并在其结束后至少持续 15 天。电子显微镜研究表明，7-OHC 诱导巨噬细胞的超微结构变化，与它们的激活以及更快地破坏摄入的沙门氏菌一致。健康和感染沙门氏菌的 7-OHC 处理动物的巨噬细胞中超氧化物和过氧化氢的分泌减少，这与目前的观点一致，即一些香豆素具有抗氧化和自由基清除活性。因此，7-OHC 预处理还通过限制氧化应激对宿主的有害组织损伤和免疫抑制作用，在沙门氏菌感染期间对宿主有益，但仍能激活暴露的吞噬细胞的杀菌能力。
