[二维奥法雷尔电泳在血液蛋白质多态性研究中的应用]
[Use of two-dimensional O'Farrell electrophoresis for studying polymorphism of blood proteins].
作者信息
Golubeva M V, Lunga I N, Shishkin S S
出版信息
Vopr Med Khim. 1991 May-Jun;37(3):64-7.
PMID:1719699
Abstract
Blood plasma of 50 healthy volunteers was analyzed using two-dimensional electrophoresis by O'Farrell. After staining with Coomassie R-250 and subsequent silver treatment of gels 200 protein fractions were detected on typical electrophoregrams. About 20 groups, belonging to known polymorphic systems, were identified. Dissimilar types of proteins electrophoretic variants were found in the preparations studied.
摘要
使用奥法雷尔二维电泳法对50名健康志愿者的血浆进行了分析。用考马斯亮蓝R-250染色并对凝胶进行后续银处理后，在典型电泳图上检测到200个蛋白质组分。鉴定出约20组属于已知多态性系统的组分。在所研究的制剂中发现了不同类型的蛋白质电泳变体。
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