Spring F A
South Western Regional Transfusion Centre, Bristol, UK.
Vox Sang. 1991;61(1):65-8. doi: 10.1111/j.1423-0410.1991.tb00930.x.
Immunoblotting with two examples of anti-Dha to the electrophoretically separated components of antigen-positive membranes gave a positive reaction with a component of the same apparent Mr (40,000) as sialoglycoprotein beta (SGP beta, syn: glycoconnectin, glycophorin C). The Dha antigenic determinant was sensitive to trypsin, but resistant to chymotrypsin and Endo F. By immunoblotting, one anti-Dha failed to react with sialidase-treated Dh(a+) cells, whilst the other gave a positive result. In contrast, neither antibody agglutinated sialidase-treated red cells. SGP beta was precipitated from Dh(a+) and Dh(a-) phenotype red cells by monoclonal anti-beta (NBTS/BRIC 10). SGP beta from Dh(a+) but not from Dh(a-) red cells was stained by immunoblotting with anti-Dha. These results assign the Dha antigenic epitope to SGP beta.
用两种抗-Dha对抗原阳性膜经电泳分离的成分进行免疫印迹，结果显示与一种表观分子量（40,000）与唾液酸糖蛋白β（SGPβ，同义词：糖连接蛋白、血型糖蛋白C）相同的成分发生阳性反应。Dha抗原决定簇对胰蛋白酶敏感，但对胰凝乳蛋白酶和内切糖苷酶F有抗性。通过免疫印迹，一种抗-Dha与经唾液酸酶处理的Dh(a+)细胞不发生反应，而另一种则呈阳性结果。相反，两种抗体均不凝集经唾液酸酶处理的红细胞。单克隆抗-β（NBTS/BRIC 10）可从Dh(a+)和Dh(a-)表型红细胞中沉淀出SGPβ。用抗-Dha进行免疫印迹时，来自Dh(a+)红细胞而非Dh(a-)红细胞的SGPβ被染色。这些结果将Dha抗原表位定位到SGPβ上。
