通过1H NMR光谱对高铁血红蛋白1和4的高自旋形式的铁血红素共振进行归属：与从X射线晶体学获得的结构数据进行比较。
Assignment of the ferriheme resonances of the high-spin forms of nitrophorins 1 and 4 by 1H NMR spectroscopy: comparison to structural data obtained from X-ray crystallography.
作者信息
Shokhireva Tatiana Kh, Smith Kevin M, Berry Robert E, Shokhirev Nikolai V, Balfour Celia A, Zhang Hongjun, Walker F Ann
机构信息
Department of Chemistry, The University of Arizona, Tucson, Arizona 85721-0041, USA.
出版信息
Inorg Chem. 2007 Jan 8;46(1):170-8. doi: 10.1021/ic061407t.
Abstract
In this work, we report the assignment of the majority of the ferriheme resonances of high-spin nitrophorins (NPs) 1 and 4 and compare them to those of NP2, published previously. It is found that the structures of the ferriheme complexes of NP1 and NP4, in terms of the orientation of the histidine imidazole ligand, can be described with good accuracy by NMR techniques and that the angle plot proposed previously for the high-spin form of the NPs (Shokhireva, T. Kh.; Shokhirev, N. V.; Walker, F. A. Biochemistry 2003, 42, 679-693) describes the angle of the effective nodal plane of the axial histidine imidazole in solution. There is an equilibrium between the two heme orientations (A and B), which depends on the heme cavity shape, which can be altered by mutation of amino acids with side chains (phenyl vs tyrosyl) near the potential position where a heme vinyl group would be in one of the isomers. The A:B ratio can be much more accurately measured by NMR spectroscopy than by X-ray crystallography.
摘要
在本研究中，我们报道了高自旋硝普蛋白（NPs）1和4的大多数高铁血红素共振峰的归属，并将它们与之前发表的NP2的共振峰进行比较。研究发现，就组氨酸咪唑配体的取向而言，NP1和NP4的高铁血红素配合物的结构可以通过核磁共振技术精确描述，并且先前为NPs的高自旋形式提出的角度图（Shokhireva, T. Kh.; Shokhirev, N. V.; Walker, F. A. Biochemistry 2003, 42, 679 - 693）描述了溶液中轴向组氨酸咪唑有效节面的角度。两种血红素取向（A和B）之间存在平衡，这取决于血红素腔的形状，而血红素腔的形状可通过在潜在位置附近具有侧链（苯基与酪氨酰基）的氨基酸突变来改变，在其中一种异构体中该潜在位置会有一个血红素乙烯基。通过核磁共振光谱法比通过X射线晶体学能够更准确地测量A:B比率。
相似文献
1
Assignment of the ferriheme resonances of the high-spin forms of nitrophorins 1 and 4 by 1H NMR spectroscopy: comparison to structural data obtained from X-ray crystallography.通过1H NMR光谱对高铁血红蛋白1和4的高自旋形式的铁血红素共振进行归属：与从X射线晶体学获得的结构数据进行比较。
Inorg Chem. 2007 Jan 8;46(1):170-8. doi: 10.1021/ic061407t.
2
Assignment of the ferriheme resonances of the low-spin complexes of nitrophorins 1 and 4 by (1)H and (13)C NMR spectroscopy: comparison to structural data obtained from X-ray crystallography.通过氢核磁共振（1H）和碳核磁共振（13C）光谱法对亲硝素1和4的低自旋配合物的高铁血红素共振进行归属：与从X射线晶体学获得的结构数据进行比较
Inorg Chem. 2007 Mar 19;46(6):2041-56. doi: 10.1021/ic061408l. Epub 2007 Feb 10.
3
Assignment of heme resonances and determination of the electronic structures of high- and low-spin nitrophorin 2 by 1H and 13C NMR spectroscopy: an explanation of the order of heme methyl resonances in high-spin ferriheme proteins.通过1H和13C NMR光谱对高铁血红蛋白2的高自旋和低自旋形式进行血红素共振归属及电子结构测定：对高自旋铁血红素蛋白中血红素甲基共振顺序的解释
Biochemistry. 2003 Jan 28;42(3):679-93. doi: 10.1021/bi026765w.
4
Assignment of the ferriheme resonances of high- and low-spin forms of the symmetrical hemin-reconstituted nitrophorins 1-4 by 1H and 13C NMR spectroscopy: the dynamics of heme ruffling deformations.通过1H和13C NMR光谱对对称血红素重构的嗜中性粒细胞明胶蛋白1-4的高自旋和低自旋形式的高铁血红素共振进行归属：血红素褶皱变形的动力学
J Biol Inorg Chem. 2008 Aug;13(6):941-59. doi: 10.1007/s00775-008-0381-8. Epub 2008 May 6.
5
1H and 13C NMR spectroscopic studies of the ferriheme resonances of three low-spin complexes of wild-type nitrophorin 2 and nitrophorin 2(V24E) as a function of pH.对野生型亲硝素2和亲硝素2（V24E）的三种低自旋配合物的高铁血红素共振进行¹H和¹³C核磁共振光谱研究，研究其作为pH值的函数关系。
J Biol Inorg Chem. 2009 Sep;14(7):1077-95. doi: 10.1007/s00775-009-0551-3. Epub 2009 Jun 11.
6
A one-residue switch reverses the orientation of a heme b cofactor. Investigations of the ferriheme NO transporters nitrophorin 2 and 7 from the blood-feeding insect Rhodnius prolixus.一个单残基开关可逆转血红素b辅因子的方向。对吸血昆虫罗阿丝虫的高铁血红素一氧化氮转运蛋白2和7的研究。
Biochemistry. 2009 Jan 20;48(2):235-41. doi: 10.1021/bi8020229.
7
Insertion of an H-bonding residue into the distal pocket of the ferriheme protein nitrophorin 4: effect on nitrite-iron coordination and nitrite disproportionation.将氢键供体残基插入亚铁血红素蛋白硝酸还原蛋白 4 的远端口袋中：对亚硝酸盐-铁配位和亚硝酸盐歧化的影响。
Chem Biodivers. 2012 Sep;9(9):1761-75. doi: 10.1002/cbdv.201100401.
8
Assignment of the 1H NMR resonances of protein residues in close proximity to the heme of the nitrophorins: similarities and differences among the four proteins from the saliva of the adult blood-sucking insect Rhodnius prolixus.分配与硝酸还原蛋白血红素近距离接近的蛋白质残基的 1H NMR 共振：来自成年吸血昆虫 Rbodnius prolixus 唾液中的四种蛋白质之间的相似性和差异。
J Biol Inorg Chem. 2012 Aug;17(6):911-26. doi: 10.1007/s00775-012-0908-x. Epub 2012 Jun 19.
9
NMR investigations of nitrophorin 2 belt side chain effects on heme orientation and seating of native N-terminus NP2 and NP2(D1A).NMR 研究硝酸酯结合蛋白 2 带侧链对血红素取向和天然 N 端 NP2 和 NP2(D1A)的座位的影响。
J Biol Inorg Chem. 2014 Jun;19(4-5):577-93. doi: 10.1007/s00775-013-1063-8. Epub 2013 Nov 30.
10
Effect of mutation of carboxyl side-chain amino acids near the heme on the midpoint potentials and ligand binding constants of nitrophorin 2 and its NO, histamine, and imidazole complexes.血红素附近羧基侧链氨基酸突变对亲硝素2及其一氧化氮、组胺和咪唑配合物的中点电位和配体结合常数的影响。
J Am Chem Soc. 2009 Feb 18;131(6):2313-27. doi: 10.1021/ja808105d.
引用本文的文献
1
Dimerization of nitrophorin 4 at low pH and comparison to the K1A mutant of nitrophorin 1.嗜镍蛋白4在低pH值下的二聚化及其与嗜镍蛋白1的K1A突变体的比较。
Biochemistry. 2015 Jan 20;54(2):208-20. doi: 10.1021/bi5013047. Epub 2014 Dec 23.
2
NMR investigations of nitrophorin 2 belt side chain effects on heme orientation and seating of native N-terminus NP2 and NP2(D1A).NMR 研究硝酸酯结合蛋白 2 带侧链对血红素取向和天然 N 端 NP2 和 NP2(D1A)的座位的影响。
J Biol Inorg Chem. 2014 Jun;19(4-5):577-93. doi: 10.1007/s00775-013-1063-8. Epub 2013 Nov 30.
3
Electron spin density on the axial His ligand of high-spin and low-spin nitrophorin 2 probed by heteronuclear NMR spectroscopy.利用异核 NMR 光谱研究高自旋和低自旋硝基蛋白 2 轴向 His 配体上的电子自旋密度。
Inorg Chem. 2013 Feb 4;52(3):1285-95. doi: 10.1021/ic301805y. Epub 2013 Jan 17.
4
Native N-terminus nitrophorin 2 from the kissing bug: similarities to and differences from NP2(D1A).原生吻盲蝽 N 端硝呋林 2：与 NP2(D1A)的相似性和差异性。
Chem Biodivers. 2012 Sep;9(9):1739-55. doi: 10.1002/cbdv.201100449.
5
Assignment of the 1H NMR resonances of protein residues in close proximity to the heme of the nitrophorins: similarities and differences among the four proteins from the saliva of the adult blood-sucking insect Rhodnius prolixus.分配与硝酸还原蛋白血红素近距离接近的蛋白质残基的 1H NMR 共振：来自成年吸血昆虫 Rbodnius prolixus 唾液中的四种蛋白质之间的相似性和差异。
J Biol Inorg Chem. 2012 Aug;17(6):911-26. doi: 10.1007/s00775-012-0908-x. Epub 2012 Jun 19.
6
NMR studies of nitrophorin distal pocket side chain effects on the heme orientation and seating of NP2 as compared to NP1.NMR 研究硝基磷蛋白远端口袋侧链对血红素取向和 NP2 与 NP1 相比的座位的影响。
J Inorg Biochem. 2011 Sep;105(9):1238-57. doi: 10.1016/j.jinorgbio.2011.06.001. Epub 2011 Jun 17.
7
1H and 13C NMR spectroscopic studies of the ferriheme resonances of three low-spin complexes of wild-type nitrophorin 2 and nitrophorin 2(V24E) as a function of pH.对野生型亲硝素2和亲硝素2（V24E）的三种低自旋配合物的高铁血红素共振进行¹H和¹³C核磁共振光谱研究，研究其作为pH值的函数关系。
J Biol Inorg Chem. 2009 Sep;14(7):1077-95. doi: 10.1007/s00775-009-0551-3. Epub 2009 Jun 11.
8
Assignment of Ferriheme Resonances for High- and Low-Spin Forms of Nitrophorin 3 by H and C NMR Spectroscopy and Comparison to Nitrophorin 2: Heme Pocket Structural Similarities and Differences.通过氢核磁共振光谱和碳核磁共振光谱对嗜铁蛋白3的高自旋和低自旋形式的高铁血红素共振进行归属，并与嗜铁蛋白2进行比较：血红素口袋结构的异同
Inorganica Chim Acta. 2008 Mar 3;361(4):925-940. doi: 10.1016/j.ica.2007.05.044.
9
Effect of mutation of carboxyl side-chain amino acids near the heme on the midpoint potentials and ligand binding constants of nitrophorin 2 and its NO, histamine, and imidazole complexes.血红素附近羧基侧链氨基酸突变对亲硝素2及其一氧化氮、组胺和咪唑配合物的中点电位和配体结合常数的影响。
J Am Chem Soc. 2009 Feb 18;131(6):2313-27. doi: 10.1021/ja808105d.
10
Assignment of the ferriheme resonances of high- and low-spin forms of the symmetrical hemin-reconstituted nitrophorins 1-4 by 1H and 13C NMR spectroscopy: the dynamics of heme ruffling deformations.通过1H和13C NMR光谱对对称血红素重构的嗜中性粒细胞明胶蛋白1-4的高自旋和低自旋形式的高铁血红素共振进行归属：血红素褶皱变形的动力学
J Biol Inorg Chem. 2008 Aug;13(6):941-59. doi: 10.1007/s00775-008-0381-8. Epub 2008 May 6.
本文引用的文献
1
Effect of heme orientation on the reduction potential of cytochrome b5.血红素取向对细胞色素b5还原电位的影响。
J Am Chem Soc. 1988 Aug 1;110(18):6234-40. doi: 10.1021/ja00226a045.
2
The heme environment of mouse neuroglobin: histidine imidazole plane orientations obtained from solution NMR and EPR spectroscopy as compared with X-ray crystallography.小鼠神经球蛋白的血红素环境：通过溶液核磁共振和电子顺磁共振光谱获得的组氨酸咪唑平面取向与X射线晶体学的比较。
J Biol Inorg Chem. 2006 Jun;11(4):391-7. doi: 10.1007/s00775-006-0095-8. Epub 2006 Apr 4.
3
Oligomerization of nitrophorins.嗜铁素的寡聚化。
Anal Biochem. 2006 May 15;352(2):286-95. doi: 10.1016/j.ab.2006.02.003. Epub 2006 Mar 20.
4
Ultrahigh resolution structures of nitrophorin 4: heme distortion in ferrous CO and NO complexes.嗜铁蛋白4的超高分辨率结构：亚铁一氧化碳和一氧化氮复合物中的血红素畸变
Biochemistry. 2005 Sep 27;44(38):12690-9. doi: 10.1021/bi0506573.
5
Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein.血红素辅助一氧化氮转运蛋白中近端硫醇盐的S-亚硝基化。
Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):594-9. doi: 10.1073/pnas.0406549102. Epub 2005 Jan 6.
6
Nitric oxide interaction with insect nitrophorins and thoughts on the electron configuration of the {FeNO}6 complex.一氧化氮与昆虫高铁血红蛋白的相互作用以及对{FeNO}6配合物电子构型的思考。
J Inorg Biochem. 2005 Jan;99(1):216-36. doi: 10.1016/j.jinorgbio.2004.10.009.
7
Protein functional cycle viewed at atomic resolution: conformational change and mobility in nitrophorin 4 as a function of pH and NO binding.在原子分辨率下观察到的蛋白质功能循环：嗜硝基蛋白4中构象变化和迁移率与pH值及一氧化氮结合的关系
Biochemistry. 2004 Nov 2;43(43):13637-47. doi: 10.1021/bi0483155.
8
Recognition of anionic phospholipid membranes by an antihemostatic protein from a blood-feeding insect.一种吸血昆虫的抗止血蛋白对阴离子磷脂膜的识别。
Biochemistry. 2004 Jun 8;43(22):6987-94. doi: 10.1021/bi049655t.
9
The structure of murine neuroglobin: Novel pathways for ligand migration and binding.小鼠神经球蛋白的结构：配体迁移与结合的新途径。
Proteins. 2004 Jul 1;56(1):85-92. doi: 10.1002/prot.20113.
10
Role of binding site loops in controlling nitric oxide release: structure and kinetics of mutant forms of nitrophorin 4.结合位点环在控制一氧化氮释放中的作用：嗜硝基蛋白4突变体形式的结构与动力学
Biochemistry. 2004 Jun 1;43(21):6679-90. doi: 10.1021/bi049748a.
Zotero 插件