用于同时检测16种与尿道炎相关病原体以诊断混合感染的系统。
System for simultaneous detection of 16 pathogens related to urethritis to diagnose mixed infection.
作者信息
Masue Naruyasu, Deguchi Takashi, Yokoi Shigeaki, Yamada Tohru, Ohkusu Kiyofumi, Ezaki Takayuki
机构信息
Department of Urology, Gifu University, Graduate School of Medicine, Gifu, Japan.
出版信息
Int J Urol. 2007 Jan;14(1):39-42. doi: 10.1111/j.1442-2042.2006.01660.x.
OBJECTIVE
Urethritis is not always caused by a single pathogen, and isolation of more than two pathogens from one patient is not uncommon. We developed a method to simultaneously detect 16 pathogens related to urethritis.
METHODS
We designed specific primers used for amplification of urethritis pathogens in our 16-well microplate assay. Sixteen microliters of each reaction mixture containing template DNA was added to each well to amplify 16 pathogens simultaneously.
RESULTS
After we evaluated the specificity and sensitivity of this microplate polymerase chain reaction method, we used it to detect pathogens in clinical samples. Of 163 clinical samples, 49.7% (81/163) were positive for specific pathogens, and 6.7% (11/163) showed mixed infection. A specific pathogen was not identified in 43.6% (71/163) of cases.
CONCLUSIONS
We developed a 16-well microplate assay with 16 specific primers to identify pathogens associated with urethritis.
目的
尿道炎并非总是由单一病原体引起，从一名患者中分离出两种以上病原体的情况并不罕见。我们开发了一种同时检测16种与尿道炎相关病原体的方法。
方法
我们设计了用于在16孔微孔板检测中扩增尿道炎病原体的特异性引物。将含有模板DNA的每个反应混合物16微升加入到每个孔中，以同时扩增16种病原体。
结果
在评估了这种微孔板聚合酶链反应方法的特异性和敏感性后，我们用它来检测临床样本中的病原体。在163份临床样本中，49.7%（81/163）的样本存在特定病原体阳性，6.7%（11/163）的样本显示混合感染。43.6%（71/163）的病例未鉴定出特定病原体。
结论
我们开发了一种带有16种特异性引物的16孔微孔板检测方法，以鉴定与尿道炎相关的病原体。
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