两种解脲支原体实时 PCR 检测方法的比较。
Comparison of two Mycoplasma genitalium real-time PCR detection methodologies.
机构信息
Department of Microbiology & Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia.
出版信息
J Clin Microbiol. 2011 Mar;49(3):1140-2. doi: 10.1128/JCM.02328-10. Epub 2011 Jan 5.
Abstract
Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).
摘要
建立了用于检测支原体属黏附蛋白（MgPa）和 16S rRNA 基因的内部定量 PCR（qPCR）检测方法，这些方法在筛查目的方面具有可比性，kappa 值为 0.97（95%置信区间 [CI]，0.94 至 1.01），定量细菌负荷无差异（P = 0.4399）。
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