Censarek Petra, Bobbe Anett, Grandoch Maria, Schrör Karsten, Weber Artur-Aron
Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany.
Thromb Haemost. 2007 Jan;97(1):11-4.
It has been proposed that alternatively-spliced human tissue factor (asHTF) is pro-coagulant. We have evaluated the function of asHTF in a mammalian expression system. Full-length human tissue factor (HTF) and asHTF were cloned from smooth muscle cells and over-expressed in HEK293 cells. As expected, a marked pro-coagulant activity (FX activation, thrombin generation) was observed on the surface, in lysates, and on microparticles from HTF transfected cells. In contrast, no pro-coagulant activity of asHTF was observed.
有人提出，可变剪接的人组织因子（asHTF）具有促凝血作用。我们在哺乳动物表达系统中评估了asHTF的功能。从平滑肌细胞中克隆了全长人组织因子（HTF）和asHTF，并在HEK293细胞中过表达。正如预期的那样，在转染HTF的细胞的表面、裂解物和微粒上观察到显著的促凝血活性（FX激活、凝血酶生成）。相比之下，未观察到asHTF的促凝血活性。
