分化剂对人卵巢腺癌细胞（OVCA 433）中CA 125、碱性磷酸酶和细胞角蛋白表达的影响。

Effect of differentiation agents on expression of CA 125, alkaline phosphatase, and cytokeratins in human ovarian adenocarcinoma cells (OVCA 433).

作者信息

Brooks S E, Timmerman J, Lau C C, Tsao S W, Knapp R C, Sheets E E

机构信息

Division of Gynecologic Oncology, Brigham and Women's Hospital, Boston, Massachusetts 02115.

出版信息

Gynecol Oncol. 1991 Sep;42(3):265-72. doi: 10.1016/0090-8258(91)90356-a.

DOI:10.1016/0090-8258(91)90356-a

PMID:1720110

Abstract

A number of chemical agents have been found to influence the proliferation, morphology, enzymatic activity, and antigen expression of neoplastic cells toward a more differentiated phenotype. We studied the effects of differentiating agents retinoic acid, sodium butyrate, and dibutyryl cyclic AMP on the expression of the tumor-associated antigen CA 125 and several biochemical markers of differentiation in cultured OVCA 433 ovarian cancer cells. Treatment of OVCA 433 cells with these agents for 96 hr reduced cellular proliferation and altered cellular morphology. Quantitation of cell surface CA 125 using flow cytometry revealed that CA 125 expression was reduced by 35-50%. The amount of CA 125 antigen shed into the culture media was reduced to a similar degree. In addition, differentiation inducers markedly enhanced cellular alkaline phosphatase activity and induced the expression of a 65-67-kDa cytokeratin. These findings provide support for the induction of a more differentiated phenotype by these agents.

摘要

已发现多种化学试剂可影响肿瘤细胞的增殖、形态、酶活性和抗原表达，使其向更分化的表型转变。我们研究了分化剂维甲酸、丁酸钠和二丁酰环磷腺苷对肿瘤相关抗原CA 125表达以及培养的OVCA 433卵巢癌细胞中几种分化生化标志物的影响。用这些试剂处理OVCA 433细胞96小时可降低细胞增殖并改变细胞形态。使用流式细胞术对细胞表面CA 125进行定量分析显示，CA 125表达降低了35 - 50%。释放到培养基中的CA 125抗原量也有类似程度的减少。此外，分化诱导剂显著增强了细胞碱性磷酸酶活性，并诱导了一种65 - 67 kDa细胞角蛋白的表达。这些发现为这些试剂诱导更分化表型提供了支持。