Fujii Kei, Sato Hiroki, Kakumoto Chiharu, Kobayashi Mari, Saito Sachiko, Kariya Tatsuya, Watanabe Yukiko, Sakoda Yoshihiro, Kai Chieko, Kida Hiroshi, Suzuki Masatsugu
Laboratory of Wildlife Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Jpn J Vet Res. 2006 Nov;54(2-3):109-17.
Serological analysis was performed to detect morbillivirus infection in Kuril harbor seals in Hokkaido, Japan. Serum samples were collected from the seals at Nosappu (231 sera), Akkeshi (16), and Erimo (75) between 1998 and 2005. Antibodies to phocine distemper virus (PDV) were detected by ELISA in seals from Nosappu and Erimo. Antibodies to PDV were found in 56% (5/9) of the sampled seals from Nosappu in 1998, versus only 5% (3/66) for 2003, 1% (1/79) for 2004, and 1% (1/77) for 2005. These suggest epidemic caused by the virus in or before 1998. As antibody-positive seals included juvenile seals in 2003 and 2005, sporadic infections of the virus are thought to have occurred in recent years. In Erimo, antibodies to PDV were found in 50% (14/28) of sampled seals in 2004, versus only 13% (1/8) for 1999, 7% (1/15) for 2003, and 0% (0/24) for 2005. These suggest sporadic infection by the virus before 2003 and the epizootic between after autumn in 2003, when samples of 2003 were collected, and 2004. Since antibodies to canine distemper virus (CDV) were detected in one adult seal from Nosappu in each year from 2003 to 2005, sporadic infections of the virus were suggested. There were no difference in incidence of seals with antibodies to the viruses between males and females and between juveniles and adults.
进行了血清学分析，以检测日本北海道千岛海豹中的麻疹病毒感染情况。1998年至2005年期间，从诺萨普（231份血清）、厚岸（16份）和襟裳（75份）的海豹身上采集了血清样本。通过酶联免疫吸附测定法（ELISA）在来自诺萨普和襟裳的海豹中检测到了抗海豹瘟热病毒（PDV）抗体。1998年，在诺萨普采集的样本海豹中有56%（5/9）检测到抗PDV抗体，而2003年只有5%（3/66），2004年为1%（1/79），2005年为1%（1/77）。这些结果表明该病毒在1998年或之前引发了疫情。由于2003年和2005年的抗体阳性海豹中包括幼年海豹，因此认为近年来该病毒发生了零星感染。在襟裳，2004年采集的样本海豹中有50%（14/28）检测到抗PDV抗体，而1999年只有13%（1/8），2003年为7%（1/15），2005年为0%（0/24）。这些结果表明2003年之前该病毒有零星感染，以及在2003年秋季之后（2003年样本采集时）到2004年之间发生了 epizootic（此处epizootic可能有误，推测应为 epizootic outbreak，即 epizootic 爆发）。由于在2003年至2005年每年从诺萨普的一只成年海豹中检测到了抗犬瘟热病毒（CDV）抗体，因此表明该病毒有零星感染。雄性和雌性海豹以及幼年和成年海豹中病毒抗体阳性海豹的发生率没有差异。
