Kang Ok Hwa, Chae Hee-Sung, Choi Jung-Hyun, Choi Hyuk Joon, Park Pil Sang, Cho Sun Hee, Lee Go-Hoon, So Hye-Young, Choo Young Kug, Kweon Oh-Hyun, Kwon Dong-Yeul
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, Republic of Korea.
J Med Food. 2006 Winter;9(4):480-6. doi: 10.1089/jmf.2006.9.480.
Schisandra fructus has been used for treatment of cough and thirst in Korea. However, its therapeutic mechanisms remain largely unclear. To investigate the biological effect of Schisandra fructus water extract (SFWE), we examined the effect of SFWE on the phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-induced pro-inflammatory cytokine secretion in the human mast cell line HMC-1. HMC-1 cells were stimulated with PMA plus A23187 in the presence or absence of SFWE. Tumor necrosis factor (TNF)-alpha, interleukin 6 (IL-6), and granulocyte-macrophage colony-stimulating factor (GM-CSF) productions were measured by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction. Inhibitory IkappaB/nuclear factor-kappaB (NF-kappaB) expression was assessed by western blot. SFWE suppressed PMA plus A23187-induced TNF-alpha, IL-6, and GM-CSF production in dose-dependent manners. Furthermore, SFWE inhibited IkappaB degradation and NF-kappaB nuclear translocation. These results suggest that SFWE inhibits the secretion of pro-inflammatory cytokines in HMC-1 cells through blockade of IkappaB degradation and NF-kappaB activation. Taken together, these findings may help elucidate the mechanism of action of this medicine in the modulation of mast cell activation in inflammatory conditions.
在韩国，五味子已被用于治疗咳嗽和口渴。然而，其治疗机制在很大程度上仍不清楚。为了研究五味子水提取物（SFWE）的生物学效应，我们检测了SFWE对佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）和钙离子载体A23187诱导的人肥大细胞系HMC - 1中促炎细胞因子分泌的影响。在有或没有SFWE的情况下，用PMA加A23187刺激HMC - 1细胞。通过酶联免疫吸附测定和逆转录 - 聚合酶链反应测量肿瘤坏死因子（TNF）-α、白细胞介素6（IL - 6）和粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）的产生。通过蛋白质免疫印迹法评估抑制性κB / 核因子 - κB（NF - κB）的表达。SFWE以剂量依赖性方式抑制PMA加A23187诱导的TNF - α、IL - 6和GM - CSF的产生。此外，SFWE抑制κB降解和NF - κB核转位。这些结果表明，SFWE通过阻断κB降解和NF - κB激活来抑制HMC - 1细胞中促炎细胞因子的分泌。综上所述，这些发现可能有助于阐明这种药物在炎症条件下调节肥大细胞活化的作用机制。
