Pérez-Pérez Elizabeth M, Rodríguez-Malaver Antonio J, Padilla Nayalet, Medina-Ramírez Gerardo, Dávila Juan
Laboratorio de Bioquímica Adaptativa, Departamento de Bioquímica, Facultad de Medicina, Universidad de Los Andes, Mérida, Mérida, Venezuela.
J Med Food. 2006 Winter;9(4):517-23. doi: 10.1089/jmf.2006.9.517.
Banana and plane are the most important fruits in world trade, behind citric plants. In this work we studied the antioxidant capacity of banana and plane varieties of fruits obtained from interspecies crossed varieties of Musa acuminata and Musa balbisiana, named Harton plane, Cavendish banana, and Manzano banana. With this purpose we evaluated banana and plane crude extracts using the ferrous ion oxidation with xylenol orange method, the thiobarbituric acid method, determination of antioxidant activity, and effect on superoxide anion and hydroxyl radical and the radicals generated by ultraviolet light. The experiments showed that all extracts have the capacity to decrease the concentrations of lipid hydroperoxides and malondialdehyde, produced in the lipid peroxidation process, in a manner comparable to that of other widely studied antioxidants like melatonin and vitamin E. Moreover, all extracts had the capacity to inhibit the generation of superoxide anion, hydroxyl radical, and the radicals generated by ultraviolet light. When antioxidant activity was calculated, a value was found that was equivalent to a concentration of uric acid between 0.20 and 0.30 mM at the highest concentration of extract used, with uric acid being a potent antioxidant at 1 mM.
香蕉和番荔枝是世界贸易中仅次于柑橘类植物的最重要水果。在这项工作中,我们研究了从尖叶蕉(Musa acuminata)和野蕉(Musa balbisiana)种间杂交品种获得的番荔枝和香蕉品种果实的抗氧化能力,这些品种分别是哈顿番荔枝、卡文迪什香蕉和苹果蕉。为此,我们使用二甲酚橙亚铁离子氧化法、硫代巴比妥酸法、抗氧化活性测定法以及对超氧阴离子、羟基自由基和紫外线产生的自由基的影响,来评估香蕉和番荔枝的粗提物。实验表明,所有提取物都有能力降低脂质过氧化过程中产生的脂质氢过氧化物和丙二醛的浓度,其降低方式与其他广泛研究的抗氧化剂如褪黑素和维生素E相当。此外,所有提取物都有能力抑制超氧阴离子、羟基自由基和紫外线产生的自由基的生成。在计算抗氧化活性时,在所使用的提取物最高浓度下,发现其值相当于尿酸浓度在0.20至0.30 mM之间,而尿酸在1 mM时是一种有效的抗氧化剂。