Darsini D Teepica Priya, Maheshu V, Vishnupriya M, Sasikumar J M
Department of Biotechnology, School of Life Sciences, Karpagam University, Coimbatore - 641 021, Tamil Nadu, India.
Indian J Biochem Biophys. 2012 Apr;49(2):124-9.
The methanolic extract of Musa ABB cv Pisang Awak was investigated for the polyphenolic contents and antioxidant activity. The total phenol and flavonoid contents of the fruit extract were found to be 120 mg gallic acid equivalents (GAE) and 440 mg quercetin equivalents (QE)/100 g of sample dry weight, respectively. The antioxidant activity of the Pisang Awak methanol extract (PAME) (20-500 microg/ml) was determined using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing capacity, 2-2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) radical cation decolourization and hydroxyl radical scavenging capacity (OH*). The EC50 values of DPPH, ABTS and OH* activities of the PAME and butylated hydroxy toluene (BHT) were found to be 65 and 9 microg/ml, 29 and 6 microg/ml, 36 and 42 microg/ml respectively. The reducing capacity increased with increasing concentration (31.5-1000 mg/ml) of the fruit extract and the activity was comparable with the standard BHT. The high performance thin layer chromatography (HPTLC) analysis of the extract revealed the presence of polyphenols. The strong and positive correlations were obtained between total phenol/flavonoid contents (R2 = 0.693-1.0) and free radical scavenging ability was attributed to the polyphenols as the major antioxidants.
对香蕉品种巴西大蕉的甲醇提取物进行了多酚含量和抗氧化活性研究。发现该水果提取物的总酚和黄酮含量分别为120毫克没食子酸当量（GAE）和440毫克槲皮素当量（QE）/100克样品干重。使用1,1-二苯基-2-苦基肼（DPPH）自由基清除活性、还原能力、2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基阳离子脱色和羟基自由基清除能力（OH*）测定了巴西大蕉甲醇提取物（PAME）（20 - 500微克/毫升）的抗氧化活性。发现PAME和丁基化羟基甲苯（BHT）的DPPH、ABTS和OH*活性的EC50值分别为65和9微克/毫升、29和6微克/毫升、36和42微克/毫升。随着水果提取物浓度（31.5 - 1000毫克/毫升）的增加，还原能力增强，且该活性与标准BHT相当。提取物的高效薄层色谱（HPTLC）分析表明存在多酚。总酚/黄酮含量（R2 = 0.693 - 1.0）与自由基清除能力之间存在强正相关，自由基清除能力归因于作为主要抗氧化剂的多酚。
