Sarson A J, Abdul-Careem M F, Zhou H, Sharif S
Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada.
Viral Immunol. 2006 Winter;19(4):747-58. doi: 10.1089/vim.2006.19.747.
This study was aimed at investigating the genes that control host responses to Marek's disease virus (MDV). Spleen tissues from infected and age-matched uninfected control chickens were examined 4, 7, 14, and 21 d postinfection for gene expression differences, using both microarray and quantitative real-time polymerase chain reaction (PCR) methodologies. Up to 51% of genes assayed during microarray analysis showed a significant change (p < or = 0.05) in expression after MDV infection, of which cell surface molecules, transcription and signal transduction molecules, housekeeping and metabolism mediators, and cytokines and cytokine receptors were most commonly differentially expressed. Setting a fold change threshold (> or =2), 14 of 84 genes showed differential expression over time. In addition, some genes showed differential expression at more than one time point. For example, the granzyme-A homolog gene remained upregulated in infected chickens, with fold changes of 7.98, 13.91, and 9.07 at 7, 14, and 21 d postinfection, respectively. Other genes that were differentially expressed at more than one time point were invariant chain, IgM, and CD3. Quantitative real-time PCR analysis was used to validate microarray results for a subset of genes showing a :2-fold change in expression. Expression of all but one gene (CD28) was confirmed. Identification of genetic mechanisms initiated by in vivo infection with MDV expands the current understanding of immune response to the virus in addition to host response elements associated with viral pathogenesis.
本研究旨在调查控制宿主对马立克氏病病毒（MDV）反应的基因。利用微阵列和定量实时聚合酶链反应（PCR）方法，在感染后4、7、14和21天检查感染鸡和年龄匹配的未感染对照鸡的脾脏组织，以寻找基因表达差异。在微阵列分析中检测的基因中，高达51%的基因在MDV感染后表达出现显著变化（p≤0.05），其中细胞表面分子、转录和信号转导分子、管家和代谢介质以及细胞因子和细胞因子受体是最常差异表达的。设定倍数变化阈值（≥2），84个基因中有14个基因随时间出现差异表达。此外，一些基因在多个时间点出现差异表达。例如，颗粒酶-A同源基因在感染鸡中持续上调，在感染后7、14和21天的倍数变化分别为7.98、13.91和9.07。在多个时间点差异表达的其他基因包括恒定链、IgM和CD3。定量实时PCR分析用于验证微阵列结果，这些结果涉及一小部分表达变化≥2倍的基因。除一个基因（CD28）外，所有基因的表达均得到证实。确定由MDV体内感染引发的遗传机制，除了与病毒发病机制相关的宿主反应元件外，还扩展了目前对该病毒免疫反应的理解。
