Digka Anna, Lyroudia Kleoniki, Jirasek Tomas, Kasampalidis Ioannis N, Karayannopoulou Georgia, Kubinova Lucie
Department of Endodontology, Dental School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Aust Endod J. 2006 Dec;32(3):101-6. doi: 10.1111/j.1747-4477.2006.00028.x.
CD34 is considered a pan-endothelial cell marker for paraffin-embedded sections. In this study, both immunohistochemistry and immunofluorescence were applied in human dental pulp specimens of moderate thickness (10 microm) in order to observe the vasculature of this tissue using CD34. Both techniques revealed a homogenous staining pattern with capillaries and larger vessels showing complete and strong membrane staining reflecting the high capacity of the pulp for regeneration and response to different stimuli. A novel approach in the identification of the pulpal vasculature by Cy5-conjugated anti-CD34 is introduced in this study. By this technique the dense capillary plexus of the sub-odontoblastic region, which is responsible for the reaction of the tissue to any physical or chemical stimuli or pathological condition, can be clearly identified, while immunohistochemistry did not reveal such a detailed staining pattern.
CD34被认为是石蜡包埋切片的全内皮细胞标志物。在本研究中，免疫组织化学和免疫荧光技术均应用于中等厚度（10微米）的人牙髓标本，以便使用CD34观察该组织的脉管系统。两种技术均显示出均匀的染色模式，毛细血管和较大血管呈现完整且强烈的膜染色，反映了牙髓的高再生能力以及对不同刺激的反应。本研究引入了一种通过Cy5偶联抗CD34鉴定牙髓脉管系统的新方法。通过该技术，可以清晰地识别成牙本质细胞下层区域的密集毛细血管丛，该区域负责组织对任何物理或化学刺激或病理状况的反应，而免疫组织化学并未显示出如此详细的染色模式。
