Institute of Anatomy and Cell Biology, University of Marburg, DE-35032 Marburg, Germany.
Cells Tissues Organs. 2013;198(1):57-65. doi: 10.1159/000351608. Epub 2013 Jun 18.
A technique for embedding human undecalcified tooth specimens in Technovit® 9100 was developed, which permits immunohistological evaluation of pulp tissue in serial ground sections. Human molars were divided into 14-18 sections of about 23 µm thickness. Immunohistological double staining for S-100 and CD34 revealed unique associations of myelinated nerve fibre bundles with arterioles, which continued through the entire tooth pulp. These arterioles were not only accompanied by, but partially or totally enveloped in longitudinally orientated myelinated nerve fibre bundles. We speculate that this unique arrangement may mechanically support the arterioles and alleviate detection or regulation of their contraction state by sensory nerve cells.
开发了一种将人未脱钙牙标本嵌入 Technovit® 9100 的技术，该技术允许对连续磨片的牙髓组织进行免疫组织化学评估。人类磨牙被分为 14-18 个约 23 µm 厚的切片。S-100 和 CD34 的免疫组织化学双重染色显示出有髓神经纤维束与小动脉的独特关联，这种关联贯穿整个牙髓。这些小动脉不仅被纵向排列的有髓神经纤维束伴行，而且部分或完全被其包裹。我们推测这种独特的排列方式可能在机械上支持小动脉，并减轻感觉神经元对其收缩状态的检测或调节。
