Foster Helen A, Stokes Paula, Forsey Katherine, Leese Henry J, Bridger Joanna M
Laboratory of Nuclear and Genomic Health, Centre for Cell and Chromosome Biology, Division of Biosciences, School of Health Sciences and Social Care, Brunel University, West London, UB8 3PH, UK.
Chromosome Res. 2007;15(2):163-74. doi: 10.1007/s10577-006-1088-8. Epub 2007 Jan 9.
Gametogenesis and embryogenesis are dynamic developmental stages marked by extensive modifications in the organization of the genome and nuclear architecture. In the literature it is conveyed that only B-type lamins are required in these early stages of development and that A-type lamins are not present or required until differentiation of specific cell types associated with specialized tissue is initiated. To assess the presence of nuclear structures that are putatively involved in genome regulation, we investigated the distribution of lamin proteins throughout the early stages of porcine embryonic development, using testes tissue sections, oocytes and in-vitro fertilized (IVF) porcine embryos and employing anti-lamin antibodies. We have shown that anti-lamin A staining is present at the one-cell, two-cell, four-cell, and six- to eight-cell stages of early porcine embryo development, but diminishes at the morulae and blastocyst stages. Large intranuclear anti-lamin A foci are prominent in the early preimplantation stages. Both anti-lamin A/C and anti-lamin B staining were clearly present in all embryonic stages. Immature porcine oocytes revealed lamin rings using the monoclonal anti-lamin A/C antibody and many immature oocytes exhibited a pale rim staining pattern with anti-lamin A antibody. A-type lamins were not observed in sperm precursor cells. Thus, we have shown that A-type lamins and B-type lamins are present at the nuclear envelope in very early porcine embryos and that lamin A is also found in large intranuclear aggregates in two-cell to eight-cell embryos but is lacking from later embryonic stages.
配子发生和胚胎发生是动态的发育阶段，其特征是基因组和核结构组织发生广泛变化。文献表明，在发育的这些早期阶段仅需要B型核纤层蛋白，而直到与特殊组织相关的特定细胞类型开始分化时，A型核纤层蛋白才会出现或被需要。为了评估可能参与基因组调控的核结构的存在情况，我们使用睾丸组织切片、卵母细胞和体外受精（IVF）的猪胚胎，并采用抗核纤层蛋白抗体，研究了核纤层蛋白在猪胚胎发育早期各阶段的分布。我们已经表明，抗A型核纤层蛋白染色在猪胚胎发育早期的单细胞、二细胞、四细胞以及六至八细胞阶段均存在，但在桑椹胚和囊胚阶段减少。在植入前早期阶段，大的核内抗A型核纤层蛋白灶很突出。抗A型/ C型核纤层蛋白和抗B型核纤层蛋白染色在所有胚胎阶段均清晰可见。未成熟的猪卵母细胞使用单克隆抗A型/ C型核纤层蛋白抗体显示出核纤层环，并且许多未成熟卵母细胞用抗A型核纤层蛋白抗体呈现出淡边缘染色模式。在精子前体细胞中未观察到A型核纤层蛋白。因此，我们已经表明，A型核纤层蛋白和B型核纤层蛋白在猪胚胎非常早期就存在于核膜，并且在二细胞至八细胞胚胎的大核内聚集体中也发现了A型核纤层蛋白，但在后期胚胎阶段则不存在。
