Houliston E, Guilly M N, Courvalin J C, Maro B
Centre de Génétique Moléculaire CNRS, Gif sur Yvette, France.
Development. 1988 Feb;102(2):271-8. doi: 10.1242/dev.102.2.271.
The expression of nuclear lamins during mouse preimplantation development was studied by immunofluorescence, immunoblotting and immunoprecipitation. Two sera were used, specific either for lamin B or lamins A and C. Both sera gave a positive staining of the nuclear periphery throughout preimplantation development (fertilized eggs to late blastocysts). Immunoblots revealed that the three lamins were present in eggs and blastocysts. However, lamin A from eggs was found to have a higher apparent Mr than lamin A from blastocysts and other mouse cells. Using immunoprecipitation, synthesis of lamin A was detected in eggs while synthesis of lamin B was detected in 8-cell embryos and blastocysts, indicating that at least some of the lamins used during early development do not come from a store in the egg. These results are discussed in relation to the possible role of lamins during cell differentiation.
通过免疫荧光、免疫印迹和免疫沉淀法研究了小鼠植入前发育过程中核纤层蛋白的表达。使用了两种血清，一种对核纤层蛋白B具有特异性，另一种对核纤层蛋白A和C具有特异性。在整个植入前发育过程（受精卵至晚期囊胚）中，两种血清均使核周呈现阳性染色。免疫印迹显示，三种核纤层蛋白存在于卵和囊胚中。然而，发现来自卵的核纤层蛋白A比来自囊胚和其他小鼠细胞的核纤层蛋白A具有更高的表观分子量。利用免疫沉淀法，在卵中检测到核纤层蛋白A的合成，而在8细胞胚胎和囊胚中检测到核纤层蛋白B的合成，这表明早期发育过程中使用的至少一些核纤层蛋白并非来自卵中的储存库。结合核纤层蛋白在细胞分化过程中可能发挥的作用对这些结果进行了讨论。
