使用声穿孔技术基于微器件递送基因产物。

Microdevice-based delivery of gene products using sonoporation.

作者信息

Siu Tung, Rohling Robert, Chiao Mu

机构信息

Department of Mechanical Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

出版信息

Biomed Microdevices. 2007 Jun;9(3):295-300. doi: 10.1007/s10544-006-9028-0.

DOI:10.1007/s10544-006-9028-0

PMID:17203382

Abstract

This paper presents a proof-of-concept miniature device for delivery of antisense oligonucleotides (ASO). A piezoelectric, lead zirconate titanate (PZT) plate (0.5 cm(2) x 0.75 mm) is used to transfect cells using cavitation-induced sonoporation. Both human umbilical vein endothelial cells (HUVEC) and human prostate cancer cells (PC3) are investigated in vitro. Preliminary results show that after sonication, the transfection rate for HUVEC increases by 96% compared to controls (p < 0.01). For PC3, the transfection rate increases by 31% compared to controls (p < 0.02). This research can potentially be applied in realizing a microelectromechanical system (MEMS)-based device for gene therapy in cancer treatment.

摘要

本文展示了一种用于递送反义寡核苷酸（ASO）的概念验证微型装置。使用压电锆钛酸铅（PZT）板（0.5平方厘米×0.75毫米）通过空化诱导的声孔效应转染细胞。在体外研究了人脐静脉内皮细胞（HUVEC）和人前列腺癌细胞（PC3）。初步结果表明，超声处理后，HUVEC的转染率与对照组相比提高了96%（p<0.01）。对于PC3，转染率与对照组相比提高了31%（p<0.02）。这项研究有可能应用于实现一种基于微机电系统（MEMS）的癌症治疗基因治疗装置。