Tanaka Yu, Koizumi Chie, Marumo Toshiyuki, Omura Tomohiro, Yoshida Shigeru
Medical Research Laboratories, Taisho Pharmaceutical Co. Ltd., 1-403 Yoshino-cho, Kita-ku, Saitama-shi, Saitama 331-9530, Japan.
Brain Res. 2007 Mar 16;1137(1):140-5. doi: 10.1016/j.brainres.2006.12.025. Epub 2007 Jan 3.
To assess the usefulness of serum S100B as a biomarker, the present study proceeded by observing serum S100B kinetics in a rat transient middle cerebral artery occlusion (MCAO) model, then assessed the correlation between serum S100B and both brain edema formation and neurological outcomes. Study results showed increases in serum S100B concentrations, peaking 48 h after MCAO. Brain water content in the ipsilateral hemisphere significantly increased from 24 h after MCAO, and reached peak value 72 h after MCAO. A single measurement of serum S100B 48 h after MCAO showed significant correlations with maximal extent of brain edema 72 h after MCAO. Furthermore, S100B concentrations 48 h after MCAO significantly correlated with infarct volumes. Neurological outcomes were estimated in a long-term study, where a gradual recovery was observed up to 168 h after MCAO. Serum S100B 48 h after MCAO was found to show higher correlation with neurological score 168 h after MCAO than those 48 h after MCAO. These findings suggest that serum S100B is an effective biomarker in predicting both extent of brain edema and long-term neurological outcomes in a rat transient MCAO model.
为评估血清S100B作为生物标志物的效用，本研究通过观察大鼠短暂性大脑中动脉闭塞（MCAO）模型中的血清S100B动力学展开，随后评估血清S100B与脑水肿形成及神经学结果之间的相关性。研究结果显示，血清S100B浓度升高，在MCAO后48小时达到峰值。同侧半球的脑含水量自MCAO后24小时起显著增加，并在MCAO后72小时达到峰值。MCAO后48小时对血清S100B进行单次测量，结果显示其与MCAO后72小时的最大脑水肿程度显著相关。此外，MCAO后48小时的S100B浓度与梗死体积显著相关。在一项长期研究中评估神经学结果，观察到MCAO后长达168小时有逐渐恢复的情况。发现MCAO后48小时的血清S100B与MCAO后168小时的神经学评分的相关性高于MCAO后48小时的相关性。这些发现表明，血清S100B是预测大鼠短暂性MCAO模型中脑水肿程度和长期神经学结果的有效生物标志物。
