Tanaka Yu, Marumo Toshiyuki, Omura Tomohiro, Yoshida Shigeru
Molecular Function and Pharmacology Laboratories, Taisho Pharmaceutical Co. Ltd., Saitama 331-9530, Japan.
Neurosci Lett. 2008 May 2;436(1):40-3. doi: 10.1016/j.neulet.2008.02.056. Epub 2008 Mar 4.
S100B is a 21-kDa, Ca(2+)-binding protein that is expressed in the central nervous system. Although the peripheral S100B level is significantly correlated with stroke outcome, the mechanisms responsible for increase in the peripheral S100B level have not been precisely investigated in animal ischemic stroke models. To justify the use of peripheral S100B as a common biomarker between stroke patients and animal models, the mechanisms responsible for increases in the peripheral S100B level after focal cerebral ischemia should be clarified. In the present study, we investigated correlations between the cerebrospinal and serum S100B levels to determine whether increase in peripheral S100B properly reflect the conditions inside the central nervous system. From each rat, cerebrospinal fluid and serum samples were collected at 24, 48, 72, or 120 h after the onset of photochemically induced thromboembolic stroke in rats. Our results indicated a difference in the kinetics of cerebrospinal and serum S100B. Among the four sampling points, the serum S100B levels were most strongly correlated with the cerebrospinal S100B levels at 48 h after PIT stroke onset. While the serum S100B level may be a useful biomarker of stroke in experimental or clinical studies, the timing of S100B measurements should be carefully selected to ensure that the serum S100B level properly reflects the conditions in the central nervous system.
S100B是一种21千道尔顿的钙结合蛋白，在中枢神经系统中表达。虽然外周血S100B水平与中风预后显著相关，但在动物缺血性中风模型中，外周血S100B水平升高的机制尚未得到精确研究。为了证明外周血S100B可作为中风患者和动物模型的通用生物标志物，应阐明局灶性脑缺血后外周血S100B水平升高的机制。在本研究中，我们调查了脑脊液和血清S100B水平之间的相关性，以确定外周血S100B的升高是否能正确反映中枢神经系统内部的情况。在大鼠光化学诱导血栓栓塞性中风发作后的24、48、72或120小时，从每只大鼠采集脑脊液和血清样本。我们的结果表明脑脊液和血清S100B的动力学存在差异。在四个采样点中，PIT中风发作后48小时血清S100B水平与脑脊液S100B水平的相关性最强。虽然血清S100B水平在实验或临床研究中可能是中风的有用生物标志物，但应仔细选择S100B测量的时间，以确保血清S100B水平能正确反映中枢神经系统的情况。
