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使用昆虫细胞培养基从患有渗出性、纤维素性心包炎的马匹中分离细菌:一项初步研究。

Use of an insect cell culture growth medium to isolate bacteria from horses with effusive, fibrinous pericarditis: a preliminary study.

作者信息

Jones Samuel L, Valenzisi Amy, Sontakke Sushama, Sprayberry Kimberly A, Maggi Ricardo, Hegarty Barbara, Breitschwerdt Edward

机构信息

Intracellular Pathogens Research Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA.

出版信息

Vet Microbiol. 2007 Mar 31;121(1-2):177-81. doi: 10.1016/j.vetmic.2006.11.024. Epub 2006 Dec 1.

DOI:10.1016/j.vetmic.2006.11.024
PMID:17204376
Abstract

Effusive, fibrinous pericarditis is an uncommon disease entity in horses. In 2001, pericarditis occurred in conjunction with an epizootic in central Kentucky that was associated with exposure to eastern tent caterpillars (ETCs). Bacterial isolation from equine pericardial fluid samples was attempted using an insect cell culture growth medium (ICCGM). Using previously cultured, stored frozen samples from four horses with fibrinous pericarditis, inoculation of 10% blood agar plates yielded no growth, whereas simultaneous inoculation of ICCGM resulted in the isolation of Proprionibacterium acnes, Staphylococcus equorum, a Streptococcus sp. and Pseudomonas rhodesiae from pericardial fluid samples. A similar or novel caterpillar-associated bacteria was not identified; however, use of an ICCGM might enhance isolation of bacteria from equine pericardial fluid.

摘要

渗出性、纤维素性心包炎在马中是一种不常见的疾病实体。2001年,肯塔基州中部发生了一起与接触东部天幕毛虫(ETC)有关的动物流行病,同时出现了心包炎。尝试使用昆虫细胞培养生长培养基(ICCGM)从马心包液样本中分离细菌。使用先前培养并冷冻保存的来自四匹患有纤维素性心包炎的马的样本,接种10%血琼脂平板未生长,而同时接种ICCGM则从心包液样本中分离出痤疮丙酸杆菌、马葡萄球菌、一种链球菌属细菌和罗得西亚假单胞菌。未鉴定出类似或新的与毛虫相关的细菌;然而,使用ICCGM可能会提高从马心包液中分离细菌的成功率。

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