Izu Y C, Sachs F
Department of Biophysical Sciences, State University of New York, Buffalo 14214.
Pflugers Arch. 1991 Sep;419(2):218-20. doi: 10.1007/BF00373012.
The ability to form gigaohm seals is essential for patch-clamp studies. Cells with otherwise useful properties must be abandoned for electrophysiological studies if seal formation is not possible. We have found that by inhibiting the growth of extracellular matrix with beta-D-xyloside, the success of forming gigaohm seals increased from near 0% to near 100%. Treated cells remained viable and appeared morphologically similar to untreated cells. Prototype treatment protocols are given for the renin secreting cell line As4.1.
形成千兆欧封接的能力对于膜片钳研究至关重要。如果无法形成封接，那么即便细胞具有其他有用的特性，也必须放弃用于电生理研究。我们发现，通过用β-D-木糖苷抑制细胞外基质的生长，形成千兆欧封接的成功率从近0%提高到了近100%。经处理的细胞仍保持活力，且形态上与未处理的细胞相似。文中给出了针对肾素分泌细胞系As4.1的原型处理方案。
