Olesen S P
NeuroSearch A/S, Glostrup, Denmark.
J Membr Biol. 1995 Apr;144(3):245-8. doi: 10.1007/BF00236837.
A cell membrane patch isolated on a patch clamp pipette incorporates in addition to the phospholipid bilayer, an extracellular matrix and cytoskeletal components. The significance of the extracellular matrix for the patch formation was studied in aortic smooth muscle and cerebellar granule cells grow in the presence of an inhibitor of proteoglycan synthesis, beta-D-xyloside. The xyloside improved the seal success rate, and after patch excision membrane vesicles were formed instead of inside-out patches. When amphotericin B was included in the pipette solution, perforated outside-out vesicles were formed in 96% of cells. The findings suggest, that membrane patches are supported by the extracellular matrix or by structures that relate to this matrix.
在膜片钳移液管上分离出的细胞膜片，除了磷脂双分子层外，还包含细胞外基质和细胞骨架成分。在主动脉平滑肌和小脑颗粒细胞中，在蛋白聚糖合成抑制剂β-D-木糖苷存在的情况下生长，研究了细胞外基质对膜片形成的意义。木糖苷提高了封接成功率，并且在膜片切除后形成了膜泡而不是内面向外的膜片。当移液管溶液中包含两性霉素B时，96%的细胞中形成了穿孔的外面向外的膜泡。这些发现表明，膜片由细胞外基质或与该基质相关的结构所支持。
