Peri K G, MacKenzie R E
Department of Biochemistry, McGill University, Montréal, Québec, Canada.
FEBS Lett. 1991 Dec 2;294(1-2):113-5. doi: 10.1016/0014-5793(91)81354-b.
The cytosolic NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase is ubiquitously expressed in all mouse tissues and cell lines examined. Northern analyses of the RNA indicated that there is an extensive variation in the levels of mRNA in different tissues. However, the gene is refractory to induction by serum, phorbol esters or growth factors in cultured fibroblasts. The mRNA of the NADP(+)-dependent trifunctional enzyme is stabilized post-transcriptionally by insulin-like growth factor-1.
胞质型烟酰胺腺嘌呤二核苷酸磷酸（NADP(+)）依赖性亚甲基四氢叶酸脱氢酶-次甲基四氢叶酸环化水解酶-甲酰四氢叶酸合成酶在所检测的所有小鼠组织和细胞系中均有广泛表达。对RNA的Northern分析表明，不同组织中mRNA水平存在广泛差异。然而，在培养的成纤维细胞中，该基因对血清、佛波酯或生长因子的诱导具有抗性。NADP(+)依赖性三功能酶的mRNA在转录后被胰岛素样生长因子-1稳定。
