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一种热休克蛋白70同源物在红藻紫菜的质体基因组上编码。

An hsp70 homolog is encoded on the plastid genome of the red alga, Porphyra umbilicalis.

作者信息

Reith M, Munholland J

机构信息

Institute for Marine Biosciences, National Research Council of Canada, Nova Scotia.

出版信息

FEBS Lett. 1991 Dec 2;294(1-2):116-20. doi: 10.1016/0014-5793(91)81355-c.

DOI:10.1016/0014-5793(91)81355-c
PMID:1720741
Abstract

A PCR experiment using Porphyra umbilicalis DNA as the template and degenerate oligonucleotides representing conserved regions of hsp70 amino acid sequences generated a 1 kb product that hybridized exclusively to the plastid DNA of this red alga. DNA sequencing of two contiguous EcoRI plastid DNA clones revealed a 620 amino acid open reading frame with 71% identity to the dnaK gene of the cyanobacterium, Synechocystis 6803. Northern hybridization experiments detected a 2.3 kb transcript that is present in control (15 degrees C) cultures and increases approximately 7-fold upon heat shock (75 minutes at 30 degrees C).

摘要

一项以紫菜DNA为模板、使用代表hsp70氨基酸序列保守区域的简并寡核苷酸进行的PCR实验产生了一个1 kb的产物，该产物仅与这种红藻的质体DNA杂交。对两个相邻的EcoRI质体DNA克隆进行DNA测序，发现一个620个氨基酸的开放阅读框，与蓝藻集胞藻6803的dnaK基因有71%的同一性。Northern杂交实验检测到一个2.3 kb的转录本，它存在于对照（15摄氏度）培养物中，在热激（30摄氏度处理75分钟）后增加约7倍。

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FEBS Lett. 1991 Dec 2;294(1-2):116-20. doi: 10.1016/0014-5793(91)81355-c.
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