Maid U, Steinmüller R, Zetsche K
Institut für Pflanzenphysiologie, Justus Liebig Universität, Giessen, Federal Republic of Germany.
Curr Genet. 1992 May;21(6):521-5. doi: 10.1007/BF00351663.
A gene homologous to the E. coli groEL locus was identified on the plastid genome of the unicellular red alga Cyanidium caldarium strain 14-1-1 (synonym: Galdieria sulphuraria). The complete nucleotide sequence was determined and compared to bacterial- and nuclear-encoded counterparts of higher plants. At the amino-acid level the C. caldarium gene shows 70% homology to the corresponding gene of the cyanobacterium Synechococcus and 52% homology to nuclear-encoded counterparts of higher plants, respectively. Northern and Western blot experiments were used to investigate the dependence of the transcript- and protein-level on culture temperature and heat shock.
在单细胞红藻嗜热栖热菌14-1-1株（同义词：嗜热栖硫菌）的质体基因组上鉴定出一个与大肠杆菌groEL基因座同源的基因。测定了其完整的核苷酸序列，并与高等植物的细菌编码和核编码对应序列进行了比较。在氨基酸水平上，嗜热栖热菌基因与蓝藻聚球藻的相应基因具有70%的同源性，与高等植物的核编码对应基因分别具有52%的同源性。采用Northern和Western印迹实验研究转录本和蛋白质水平对培养温度和热休克的依赖性。
