Vorobjeva L I, Cherdinceva T A, Abilev S K, Vorobjeva N V
Department of Microbiology, Biology Faculty, Moscow State University, U.S.S.R.
Mutat Res. 1991 Dec;251(2):233-9. doi: 10.1016/0027-5107(91)90078-3.
The antimutagenic effect of dialysed cell extracts of 4 strains of propionic acid bacteria was examined against the mutagenicity of sodium azide in the TA1535 tester strain of Salmonella typhimurium using the Ames test. It was noted that dialysates of 2 strains of Propionibacterium shermanii, P. pentosaceum and P. acnes, significantly reduced sodium azide-induced revertants. The dialysate of propionic acid cocci did not show an antimutagenic effect. The inhibitory activity was enhanced if the mutagen and extract were coincubated for 20 min prior to performing the mutagenicity assay. Antimutagenicity of dialysates from P. shermanii VKM-103 against MNNG and 9-aminoacridine was shown in S. typhimurium strains TA1535 and TA97. The antimutagenic activity was found in the protein fraction of the cell extract of P. shermanii. The proteins of the dialysate of P. shermanii were separated using a Toyopearl gel column into 3 main peaks according to their molecular weights. The antimutagenic activity towards sodium azide was found in the second and the third peaks. We suggest that dialysates of the cells of propionic acid bacteria contain several kinds of antimutagenic substances with different molecular weights.
利用艾姆斯试验，检测了4株丙酸杆菌的透析细胞提取物对鼠伤寒沙门氏菌TA1535测试菌株中叠氮化钠致突变性的抗诱变作用。值得注意的是，2株谢氏丙酸杆菌、戊糖丙酸杆菌和痤疮丙酸杆菌的透析液显著降低了叠氮化钠诱导的回复突变体数量。丙酸球菌的透析液未显示出抗诱变作用。如果在进行致突变性试验前将诱变剂和提取物预孵育20分钟，抑制活性会增强。谢氏丙酸杆菌VKM - 103透析液对鼠伤寒沙门氏菌TA1535和TA97菌株中的MNNG和9 - 氨基吖啶显示出抗诱变作用。在谢氏丙酸杆菌细胞提取物的蛋白质部分发现了抗诱变活性。谢氏丙酸杆菌透析液中的蛋白质通过Toyopearl凝胶柱根据分子量分离为3个主要峰。在第二个和第三个峰中发现了对叠氮化钠的抗诱变活性。我们认为，丙酸杆菌细胞的透析液含有几种不同分子量的抗诱变物质。
