浮萍（Spirodela polyrrhiza (L.) Schleiden）叶绿体tRNA基因trnL-trnF间隔区核苷酸序列多态性分析

[Analysis of nucleotide sequences polymorphism of chloroplast trnL-trnF spacer of tRNA genes in giant duckweed Spirodela polyrrhiza (L.) Schleiden].

作者信息

Ryzhova N N, Martirosian L V, Kolganova T V, Goriunova S V, Kochieva E Z

出版信息

Mol Biol (Mosk). 2006 Nov-Dec;40(6):989-95.

PMID:17209426

Abstract

Chloroplast DNA trnL-trnF spacer sequences of tRNA genes of 14 specimens of the fam. Lemnaceae have been characterized. Nucleotide polymorphism analysis of the spacer trnL-trnF of geographically isolated and morphologically differing accessions of S. polyrrhiza that is the most widespread species of Spirodela genus showed the low level of intraspecific variability. Five trnL-trnF haplotypes of S. polyrrhiza are identified. Both mono-, and polynucleotide repeats, and also extensive indels, specific to representatives Spirodela polyrrhiza, Landoltia punctata and Lemna sp. are revealed. Competency of Landoltia genus allocation as separate entity was confirmed.

摘要

已对浮萍科14个样本的叶绿体DNA tRNA基因的trnL-trnF间隔序列进行了特征分析。对浮萍属分布最广的物种多根紫萍在地理上隔离且形态不同的种质的trnL-trnF间隔区进行核苷酸多态性分析，结果显示种内变异性较低。已鉴定出多根紫萍的5种trnL-trnF单倍型。发现了多根紫萍、稀脉浮萍和浮萍属代表植物特有的单核苷酸和多核苷酸重复序列，以及广泛的插入缺失。确认了将稀脉浮萍属作为独立实体划分的合理性。