Martirosian E V, Ryzhova N N, Skriabin K G, Kochieva E Z
Genetika. 2008 Mar;44(3):417-22.
The multilocus RAPD analysis of intergeneric, inter- and intraspecific nuclear genome polymorphism was used for the first time to assess intergeneric, interspecific, and intraspecific polymorphism in Lemnaceae growing on the territory of Russia. The origin of the chosen accessions overlapped with the natural range of duckweeds in Russia. Seventy-five Lemnaceae accessions representing eight species (L. minor, L. gibba, L. turionifera, L. japonica. L. trisulca, L. aequinoctialis, S. polyrhiza, and L. punctata) from three genera (Lemna, Spirodela, and Landoltia), were analyzed. The highest variability levels were revealed in L. minor accessions (0.03-0.20). Species L. trisulca and S. polyrhiza were characterized by values of genetic distance 0.01-0.18 and 0.03-0.16, respectively. The lowest polymorphism levels were detected for L. turionifera (0.01-0.11). The dendrogram based on RAPD data showed that L. aequinoctialis was the most genetically distant species of the genus Lemna. Accessions of species L. turionifera and L. japonica, as well as L. minor and L. gibba, did not form separate species-specific subclusters; rather, they fell into clusters with L. japonica/L. turionifera and L. minor/L. gibba. Accessions of the genera Spirodela and Landoltia formed two separate clusters combined into one group.
首次使用多位点随机扩增多态性DNA（RAPD）分析来评估俄罗斯境内浮萍科植物属间、种间和种内的核基因组多态性。所选种质的起源与俄罗斯浮萍的自然分布范围重叠。分析了来自浮萍属（Lemna）、紫萍属（Spirodela）和青萍属（Landoltia）三个属的75份浮萍科种质，代表8个物种（小浮萍L. minor、浮苔L. gibba、瘤状浮萍L. turionifera、日本浮萍L. japonica、三裂浮萍L. trisulca、赤道浮萍L. aequinoctialis、紫萍S. polyrhiza和斑条萍L. punctata）。小浮萍种质的变异性水平最高（0.03 - 0.20）。三裂浮萍和紫萍物种的遗传距离值分别为0.01 - 0.18和0.03 - 0.16。瘤状浮萍的多态性水平最低（0.01 - 0.11）。基于RAPD数据的聚类图表明，赤道浮萍是浮萍属中遗传距离最远的物种。瘤状浮萍和日本浮萍的种质，以及小浮萍和浮苔的种质，没有形成单独的物种特异性亚簇；相反，它们与日本浮萍/瘤状浮萍和小浮萍/浮苔聚类在一起。紫萍属和青萍属的种质形成了两个单独的聚类，并合并为一组。
