Piekarz Ana V, Ward Wendy E
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada, M5S 3E2.
Pediatr Res. 2007 Jan;61(1):48-53. doi: 10.1203/01.pdr.0000250200.94611.03.
Infants fed soy-based infant formulas are exposed to high levels of genistein, an isoflavone, with potential estrogen-like activity. This study determined whether neonatal exposure of mice to genistein resulted in higher bone mineral density (BMD) and greater resistance to fracture at adulthood. Male and female CD-1 mice (n = 4-14/group) were randomized to control (CON) (corn oil, s.c.), diethylstilbestrol (DES) (2 microg/pup/d, s.c.), or genistein (GEN) (4 microg/pup/d, s.c.) from d 1 through 5 of life. At 21 d of age, pups were weaned and studied until 4 mo of age when tissues were collected. Among females, femur (p = 0.016) and lumbar vertebrae (LV1-LV4) (p < 0.001) BMD were higher among DES and GEN groups compared with CON group. Importantly, the higher LV1-LV4 BMD was associated with stronger vertebrae that were more resistant to fracture as the peak load of LV3 (p = 0.012) was higher in the GEN and DES groups compared with CON group. In males, DES and GEN had divergent effects on femur and lumbar vertebrae BMD and peak load. In conclusion, early exposure to GEN has positive effects on femur and lumbar spine of females, likely due to estrogenic effects, while only the lumbar spine of males benefits from early exposure to GEN.
食用大豆基婴儿配方奶粉的婴儿会接触到高水平的染料木黄酮，这是一种异黄酮，具有潜在的雌激素样活性。本研究确定了新生小鼠接触染料木黄酮是否会导致成年后更高的骨矿物质密度（BMD）和更强的抗骨折能力。将雄性和雌性CD-1小鼠（每组n = 4 - 14只）从出生第1天到第5天随机分为对照组（CON）（皮下注射玉米油）、己烯雌酚（DES）组（每只幼崽每天皮下注射2微克）或染料木黄酮（GEN）组（每只幼崽每天皮下注射4微克）。在21日龄时，幼崽断奶并进行研究，直至4月龄时收集组织。在雌性小鼠中，DES组和GEN组的股骨（p = 0.016）和腰椎（LV1 - LV4）（p < 0.001）骨密度高于CON组。重要的是，更高的LV1 - LV4骨密度与更强的椎骨相关，这些椎骨更能抵抗骨折，因为与CON组相比，GEN组和DES组的LV3峰值负荷更高（p = 0.012）。在雄性小鼠中，DES和GEN对股骨和腰椎骨密度以及峰值负荷有不同的影响。总之，早期接触GEN对雌性小鼠的股骨和腰椎有积极影响，可能是由于雌激素效应，而雄性小鼠只有腰椎从早期接触GEN中受益。
