Ward Wendy E, Piekarz Ana V
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Canada.
Pediatr Res. 2007 Apr;61(4):438-43. doi: 10.1203/pdr.0b013e3180332d67.
Early postnatal exposure to genistein resulted in improved bone health at early adulthood in mice. The objective of the present study was to determine whether in utero exposure to isoflavones also has a positive effect on bone health, resulting in higher bone mineral density (BMD) and greater resistance to fracture at adulthood. Pregnant mice received daily subcutaneous injections of genistein (3.75 mg), daidzein (3.75 mg), genistein (3.75 mg) + daidzein (3.75 mg), or vehicle from d 9 to 21 of pregnancy. At birth, offspring (n = 12/group/gender) remained with their respective mother and were weaned at postnatal age of 21 d and fed control diet, devoid of isoflavones, until 4 mo of age, at which time tissues were collected. There was an overall effect of treatment on femur BMD, which was higher (p<0.001) among control and genistein groups compared with daidzein and genistein + daidzein groups. Treatment did not have a significant effect on femur peak load. Among females, daidzein resulted in a lower (p=0.02) BMD of lumbar vertebra (LV) 1-4 than all other groups however peak load of LV4 did not differ due to treatment or gender. In conclusion, in utero exposure to isoflavones did not result in functional benefits to bone at young adulthood.
产后早期接触染料木黄酮可使小鼠成年早期的骨骼健康得到改善。本研究的目的是确定子宫内接触异黄酮是否也对骨骼健康有积极影响，从而在成年期产生更高的骨矿物质密度（BMD）和更强的抗骨折能力。怀孕小鼠在妊娠第9天至21天每天皮下注射染料木黄酮（3.75毫克）、大豆苷元（3.75毫克）、染料木黄酮（3.75毫克）+大豆苷元（3.75毫克）或赋形剂。出生时，后代（每组/性别n = 12）与各自的母亲在一起，在出生后21天断奶，喂食不含异黄酮的对照饮食，直至4月龄，此时收集组织。治疗对股骨BMD有总体影响，与大豆苷元和染料木黄酮+大豆苷元组相比，对照组和染料木黄酮组的股骨BMD更高（p<0.001）。治疗对股骨峰值负荷没有显著影响。在雌性中，大豆苷元导致第1 - 4腰椎（LV）的BMD低于所有其他组（p = 0.02），然而LV4的峰值负荷并未因治疗或性别而有所不同。总之，子宫内接触异黄酮在成年早期并未给骨骼带来功能益处。
