Gergova Raina T, Iankov Ianko D, Haralambieva Iana H, Mitov Ivan G
Department of Microbiology, Medical University, Sofia, Bulgaria.
Curr Microbiol. 2007 Feb;54(2):85-90. doi: 10.1007/s00284-005-0463-6. Epub 2007 Jan 5.
Monoclonal antibodies (MAbs) against lipooligosaccharide (LOS) determinants after immunization of BALB/c mice with heat inactivated Moraxella catarrhalis serotype A were generated. MAb 219A9 was specific for a common epitope of A, B, and C M. catarrhalis serotypes in ELISA and immunofluorescent test (IFT). In both tests it also cross-reacted with whole bacteria and LPS antigens isolated from non-typeable H. influenzae and H. parainfluenzae strains. IgM antibody clone 219A9 possessed a strong bactericidal effect against the three serotypes in the presence of complement. Our results demonstrate that antibodies directed to a single LOS epitope common for A, B, and C serotype could be highly protective. This suggests that the common determinants are very promising in the development of LOS-based vaccine against M. catarrhalis. The cross-reactions of MAb 219A9 with Haemophilus spp. also show that immunization could result in immune response to epitopes conserved in other important respiratory pathogens.
在用热灭活的卡他莫拉菌A血清型免疫BALB/c小鼠后，产生了针对脂寡糖（LOS）决定簇的单克隆抗体（MAb）。单克隆抗体219A9在酶联免疫吸附测定（ELISA）和免疫荧光试验（IFT）中对卡他莫拉菌A、B和C血清型的一个共同表位具有特异性。在这两种试验中，它还与从不可分型流感嗜血杆菌和副流感嗜血杆菌菌株中分离出的全菌及脂多糖（LPS）抗原发生交叉反应。IgM抗体克隆219A9在补体存在的情况下对这三种血清型具有很强的杀菌作用。我们的结果表明，针对A、B和C血清型共有的单一LOS表位的抗体可能具有高度保护作用。这表明这些共同决定簇在开发基于LOS的卡他莫拉菌疫苗方面非常有前景。单克隆抗体219A9与嗜血杆菌属的交叉反应也表明，免疫可导致对其他重要呼吸道病原体中保守表位的免疫反应。
