一种与卡他莫拉菌（布兰汉菌属）脂多糖共同表位发生反应的单克隆抗体。
A monoclonal antibody reactive with a common epitope of Moraxella (Branhamella) catarrhalis lipopolysaccharides.
作者信息
Oishi K, Tanaka H, Sonoda F, Borann S, Ahmed K, Utsunomiya Y, Watanabe K, Nagatake T, Vaneechoutte M, Verschraegen G, Matsumoto K
机构信息
Department of Internal Medicine, Nagasaki University, Japan.
出版信息
Clin Diagn Lab Immunol. 1996 May;3(3):351-4. doi: 10.1128/cdli.3.3.351-354.1996.
Abstract
A hybrid cell line producing a monoclonal antibody (MAb) against Moraxella (Branhamella) catarrhalis lipopolysaccharide (LPS) was established. The specificity of the MAb 1B12 to purified rough LPSs from six strains of M. catarrhalis was ascertained by enzyme-linked immunosorbent assay (ELISA), competitive-inhibition ELISA, and immunoblotting. MAb 1B12 bound to live bacterial cells and culture supernatants from a total of 34 strains of M. catarrhalis, including 12 strains with different LPS serotypes. No cross-reactions with smooth and rough LPSs from selected enterobacterial and nonenterobacterial strains, with other respiratory pathogens, or with Neisseria species were observed. These data suggest that MAb 1B12 recognizes a common epitope of M. catarrhalis LPS which differs from serotype determinants.
摘要
建立了一种杂交细胞系，该细胞系可产生针对卡他莫拉菌（布兰汉菌属）脂多糖（LPS）的单克隆抗体（MAb）。通过酶联免疫吸附测定（ELISA）、竞争抑制ELISA和免疫印迹法确定了单克隆抗体1B12对来自6株卡他莫拉菌的纯化粗糙LPS的特异性。单克隆抗体1B12与总共34株卡他莫拉菌的活细菌细胞和培养上清液结合，其中包括12株具有不同LPS血清型的菌株。未观察到与所选肠杆菌和非肠杆菌菌株的光滑和粗糙LPS、其他呼吸道病原体或奈瑟菌属的交叉反应。这些数据表明，单克隆抗体1B12识别卡他莫拉菌LPS的一个共同表位，该表位不同于血清型决定簇。
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