[银屑病角质形成细胞凋亡与患者病情严重程度]

[Apoptosis of psoriatic keratinocytes and the severity of patients' condition].

作者信息

Chen Jing, Lu Jian-yun, Tang Zhao-hui, Zuo Cheng-xin, Huang Jin-hua

机构信息

Department of Dermatology, Third Xiangya Hospital of Central South University, Changsha 410013, China.

出版信息

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2006 Dec;31(6):936-9.

PMID:17213601

Abstract

OBJECTIVE

To investigate the correlation between the apoptosis of psoriatic keratinocytes (KCs) in the psoriatic lesions and patient's condition.

METHODS

Thirty patients with psoriasis were included. Activated caspase-3 was examined by colorimetric method in skin biopsy specimens. The apoptotic KCs were detected with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay in skin biopsy specimens. The severities of 26 patients with psoriasis vulgaris (PV) were assessed using the PASI scoring system and the severities of psoriatic lesions.

RESULTS

Apoptotic indexes (AI) were higher in the pustular psoriatic lesions than those in the vulgaris psoriatic lesions (P<0.05). In 26 patients with PV, AI and activated caspase-3 in the lesions were significantly higher in the progressive stage than those in the static and the regressive stages (P<0.05). There were no correlations between PASI and AI or the expression of activated caspase-3 in PV lesions (P>0.05). The severities of psoriatic lesions were positively correlated to AI or the expression of activated caspase-3 in PV lesions (P<0.05).

CONCLUSION

The amount of apoptotic KCs may be related to varied types, stages of psoriasis, and severities of psoriatic lesions.

摘要

目的

探讨银屑病皮损中角质形成细胞（KCs）凋亡与患者病情之间的相关性。

方法

纳入30例银屑病患者。采用比色法检测皮肤活检标本中活化的半胱天冬酶-3。用末端脱氧核苷酸转移酶介导的dUTP生物素缺口末端标记法（TUNEL法）检测皮肤活检标本中的凋亡角质形成细胞。采用银屑病面积和严重程度指数（PASI）评分系统评估26例寻常型银屑病（PV）患者的病情严重程度及银屑病皮损的严重程度。

结果

脓疱型银屑病皮损的凋亡指数（AI）高于寻常型银屑病皮损（P<0.05）。在26例PV患者中，皮损的AI和活化的半胱天冬酶-3在进行期显著高于静止期和退行期（P<0.05）。PV皮损中PASI与AI或活化的半胱天冬酶-3表达之间无相关性（P>0.05）。PV皮损的严重程度与AI或活化的半胱天冬酶-3表达呈正相关（P<0.05）。