Takeuchi Masaki, Ozawa Takeaki
Department of Molecular Structure, Institute for Molecular Science, Okazaki, Japan.
Anal Sci. 2007 Jan;23(1):25-9. doi: 10.2116/analsci.23.25.
One of the important features of eukaryotic cells is the presence of intracellular compartments, organelles. Each organelle plays an essential role in the organelle-specific function; each cooperates with others for maintaining the vital activity of living cells. To analyze the organelle-specific functions, researchers have used many fluorescent and luminescent reporter proteins extensively. The reporter proteins have provided unprecedented insights into the movement of proteins and their interactions in the organelles. In this review, we summarize recently validated methods for visualizing protein localization, dynamics and protein-protein interactions, and for identifying a series of proteins localized in organelles using newly developed reporter proteins.
真核细胞的一个重要特征是存在细胞内区室，即细胞器。每个细胞器在特定细胞器功能中发挥着至关重要的作用；各个细胞器相互协作以维持活细胞的生命活动。为了分析细胞器的特定功能，研究人员广泛使用了许多荧光和发光报告蛋白。这些报告蛋白为蛋白质在细胞器中的移动及其相互作用提供了前所未有的见解。在本综述中，我们总结了最近经过验证的用于可视化蛋白质定位、动态变化和蛋白质-蛋白质相互作用，以及使用新开发的报告蛋白鉴定一系列定位于细胞器中的蛋白质的方法。
