Tsien Roger Y
Department of Pharmacology, University of California, San Diego, Howard Hughes Medical Institute, 310 George Palade Laboratories 0647, La Jolla, CA 92093-0647, USA.
FEBS Lett. 2005 Feb 7;579(4):927-32. doi: 10.1016/j.febslet.2004.11.025.
Imaging of biochemical processes in living cells and organisms is essential for understanding how genes and gene products work together in space and time and in health and disease. Such imaging depends crucially on indicator molecules designed to maximize sensitivity and specificity. These molecules can be entirely synthetic, entirely genetically encoded macromolecules, or hybrid combinations, each approach having its own pros and cons. Recent examples from the author's laboratory include peptides whose uptake into cells is triggered by proteases typical of tumors, monomeric red fluorescent proteins and biarsenical-tetracysteine systems for determining the age and electron-microscopic location of proteins.
对活细胞和生物体中的生化过程进行成像，对于理解基因和基因产物如何在空间和时间上以及在健康和疾病状态下协同工作至关重要。这种成像关键取决于旨在最大化灵敏度和特异性的指示分子。这些分子可以是完全合成的、完全基因编码的大分子或杂交组合，每种方法都有其优缺点。作者实验室最近的例子包括其进入细胞由肿瘤典型蛋白酶触发的肽、用于确定蛋白质年龄和电子显微镜定位的单体红色荧光蛋白和双砷 - 四半胱氨酸系统。
