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Preparation of N-acylated proteins modified with fatty acids having a specific chain length using an insect cell-free protein synthesis system.

作者信息

Suzuki Takashi, Ito Masaaki, Ezure Toru, Shikata Masamitsu, Ando Eiji, Utsumi Toshihiko, Tsunasawa Susumu, Nishimura Osamu

机构信息

Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation, Japan.

出版信息

Biosci Biotechnol Biochem. 2007 Jan;71(1):261-4. doi: 10.1271/bbb.60470. Epub 2007 Jan 7.

DOI:10.1271/bbb.60470
PMID:17213645
Abstract

To establish a strategy to generate N-acylated proteins modified with fatty acids having a specific chain length, tGelsolin-streptag, an epitope-tagged model protein having an N-myristoylation motif, was synthesized using an insect cell-free protein synthesis system in the presence of acyl-CoA with various fatty acid chain lengths. It was found that the fatty acid species attached to the N-termini fully depended on the acyl-CoA species added to the reaction mixture. N-Acylated proteins with fatty acid chain lengths of 8, 10, 12, and 14 were generated successfully.

摘要

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