Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, China.
Rapid Commun Mass Spectrom. 2011 Nov 15;25(21):3223-34. doi: 10.1002/rcm.5218.
In this work, graphene (G) and graphene oxide (GO) were utilized to enrich and ionize long-chain fatty acids. All together five long-chain fatty acids were selected as models here, n-dodecanoic acid (C12), n-tetradecanoic acid (C14), n-hexadecanoic acid (C16), n-octadecanoic acid (C18), and n-eicosanoic acid (C20). Due to the large surface area and strong interaction force of G or GO, all the five long-chain fatty models were effectively enriched by G or GO. On the other hand, the excellent electronic, thermal, and mechanical properties enable G and GO to be prefect energy receptacles for laser radiation, which make the ionization steps more effective. Eventually, the promoted G and GO methodology can sensitively detect the five long-chain fatty acid models from real biological samples even at low concentrations. Meanwhile, by adopting our promoted methodology, the detection of long-chain fatty acids by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was demonstrated to be simple, sensitive, fast, cost effective and high throughput, which is meaningful as to practical usage.
在这项工作中，石墨烯（G）和氧化石墨烯（GO）被用于富集和离子化长链脂肪酸。总共选择了五种长链脂肪酸作为模型，分别是十二烷酸（C12）、十四烷酸（C14）、十六烷酸（C16）、十八烷酸（C18）和二十烷酸（C20）。由于 G 或 GO 的表面积大且相互作用力强，所有五种长链脂肪酸模型都被 G 或 GO 有效地富集。另一方面，G 和 GO 的优异的电子、热和机械性能使其成为激光辐射的理想能量容器，这使得离子化步骤更加有效。最终，这种改进的 G 和 GO 方法能够从实际生物样本中甚至在低浓度下灵敏地检测到五种长链脂肪酸模型。同时，通过采用我们改进的方法，基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）检测长链脂肪酸变得简单、灵敏、快速、经济高效且高通量，这对于实际应用具有重要意义。
