Chowdhary Rashmi, Shukla Alka, Datta Tapas, Dhole T N
Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Arch Environ Occup Health. 2005 Jul-Aug;60(4):223-8. doi: 10.3200/AEOH.60.4.223-228.
The authors' objective in this study was to introduce and evaluate integrated cell culture polymerase chain reaction (ICC-PCR) as a technique for the rapid screening of poliovirus in sewage samples. Researchers are in the last stage of poliomyelitis eradication; however, in a densely populated country such as India, time is the prime factor in the identification of poliovirus circulation and transmission because this virus follows the fecal-oral route for transmission and is excreted in nature. The authors used ICC-PCR to detect poliovirus in sewage samples, and they compared this nonconventional method with conventional cell culture methods to determine sensitivity, accuracy, and the time from sample collection to final results. The ICC-PCR method provided results within 4-5 days of sewage-sample collection; in contrast, the conventional method takes more than 18 days to provide such results. The ICC-PCR method proved to be sensitive, reproducible, and accurate, as well as rapid in its screening of sewage samples for poliovirus. This diagnostic tool may indeed prove quite useful in polio eradication.
作者在本研究中的目的是引入并评估整合细胞培养聚合酶链反应（ICC-PCR）作为一种快速筛查污水样本中脊髓灰质炎病毒的技术。研究人员正处于根除脊髓灰质炎的最后阶段；然而，在像印度这样人口密集的国家，时间是确定脊髓灰质炎病毒传播和扩散的首要因素，因为这种病毒通过粪口途径传播且在自然界中排泄。作者使用ICC-PCR检测污水样本中的脊髓灰质炎病毒，并将这种非常规方法与传统细胞培养方法进行比较，以确定灵敏度、准确性以及从样本采集到得出最终结果所需的时间。ICC-PCR方法在污水样本采集后的4至5天内就能得出结果；相比之下，传统方法需要超过18天才能得出此类结果。ICC-PCR方法在筛查污水样本中的脊髓灰质炎病毒时，被证明具有灵敏性、可重复性和准确性，而且速度很快。这种诊断工具在根除脊髓灰质炎方面可能确实会非常有用。
