Stegmeier Johannes F, Glück Andreas, Sukumaran Suja, Mäntele Werner, Andersen Christian
Lehrstuhl für Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany.
Biol Chem. 2007 Jan;388(1):37-46. doi: 10.1515/BC.2007.004.
Omp85 proteins form a ubiquitous protein family, members of which are found in all Gram-negative bacteria. Omp85 of Neisseria meningitidis and YaeT of Escherichia coli are shown to be essential for outer membrane biogenesis. Interestingly, there exists a homologue to YaeT in E. coli and many proteobacteria, denoted YtfM, the function of which has not been described yet. Like YaeT, YtfM is predicted to consist of an amino-terminal periplasmic domain and a membrane-located carboxy-terminal domain. In this study, we present a first characterisation of YtfM by comparison to YaeT concerning structural, biochemical and electrophysiological properties. Furthermore, a knockout strain revealed that ytfM is a non-essential gene and lack of the protein had no effect on outer membrane composition and integrity. The only observable phenotype was strongly reduced growth, indicating an important role of YtfM in vivo.
Omp85蛋白构成了一个普遍存在的蛋白家族，其成员存在于所有革兰氏阴性菌中。脑膜炎奈瑟菌的Omp85和大肠杆菌的YaeT被证明对外膜生物合成至关重要。有趣的是，在大肠杆菌和许多变形菌中存在YaeT的一个同源物，称为YtfM，其功能尚未被描述。与YaeT一样，YtfM预计由一个氨基末端周质结构域和一个位于膜上的羧基末端结构域组成。在本研究中，我们通过与YaeT比较，首次对YtfM的结构、生化和电生理特性进行了表征。此外，一个基因敲除菌株表明ytfM是一个非必需基因，该蛋白的缺失对外膜组成和完整性没有影响。唯一可观察到的表型是生长强烈减少，表明YtfM在体内具有重要作用。
