Kim Kisu, Hur Youngmi, Ryu En-Kyung, Rhim Jung-Hyo, Choi Cha Yong, Baek Cheol-Min, Lee Jae-Ho, Chung Junho
Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-gu, Seoul, Republic of Korea.
Biochem Biophys Res Commun. 2007 Mar 2;354(1):115-21. doi: 10.1016/j.bbrc.2006.12.164. Epub 2006 Dec 29.
A new conformational neutralizable epitope is created on heptocyte growth factor (HGF), when it interacts with its receptor, cMet. By immunizing rabbits with HGF-cMet complex, we successfully generated a monoclonal antibody (SFN68) that inhibits HGF-cMet interaction, and blocks the biological function mediated by HGF. To define the epitope, we screened out an epitope-mimicking peptide, KSLSRHDHIHHH, from a phage display of combinatorial peptide library. In molecular mimicry this peptide bound to cMet and inhibited HGF-cMet interaction. No humoral response was induced to this epitope-mimicking peptide when immunization was done with HGF alone.
当肝细胞生长因子（HGF）与其受体cMet相互作用时，会在HGF上产生一个新的可构象中和表位。通过用HGF-cMet复合物免疫兔子，我们成功产生了一种抑制HGF-cMet相互作用并阻断HGF介导的生物学功能的单克隆抗体（SFN68）。为了确定该表位，我们从组合肽库的噬菌体展示中筛选出一个模拟表位的肽KSLSRHDHIHHH。在分子模拟中，该肽与cMet结合并抑制HGF-cMet相互作用。单独用HGF进行免疫时，不会对该模拟表位的肽诱导体液反应。
