从兔抗体库到兔单克隆抗体。
From rabbit antibody repertoires to rabbit monoclonal antibodies.
作者信息
Weber Justus, Peng Haiyong, Rader Christoph
机构信息
Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA.
出版信息
Exp Mol Med. 2017 Mar 24;49(3):e305. doi: 10.1038/emm.2017.23.
In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.
在本综述中，我们解释了兔单克隆抗体为何以及如何成为实验室研究的出色试剂，并越来越多地用于诊断和治疗应用。从兔B细胞独特的个体发育开始，其产生了高度独特的抗体库，富含体内经过筛选的具有高多样性、亲和力和特异性的结合物，我们描述了通过杂交瘤技术、噬菌体展示和其他方法产生兔单克隆抗体的过程，以及成功的人源化策略。
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