Danielisová Viera, Gottlieb Miroslav, Némethová Miroslava, Burda Jozef
Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 6, 040 01 Kosice, Slovak Republic.
J Neurol Sci. 2007 Feb 15;253(1-2):61-5. doi: 10.1016/j.jns.2006.12.001. Epub 2007 Jan 9.
The activity of SOD and CAT was measured in controls and 5 h after 5, 10 and 15 min of ischemia, as well as 1 or 2 days after 10 min of ischemia in the hippocampus and in the CSF. A significant increase in total SOD activity 5 h after ischemia was caused mainly by increased CuZn-SOD activity. The highest values were measured 5 h after 5 min ischemia (by 160%) and smallest if 15 min (by 40%) of ischemia was used. In comparison to the hippocampus, the activity of SOD in CSF increased equally after all intervals of ischemia. Activities of total SOD and CuZn-SOD after 10 min of ischemia in the hippocampus were significantly increased only after 5 and 24 h of reperfusion but in CSF they were increased after all examined intervals of reperfusion. The activity of CAT was significantly increased in the hippocampus after 5 (by 260%), 10 and 15 min (by 100%) of ischemia. CAT activity in CSF was increased equally after all intervals of ischemia (by 200%). Ischemic attack causes a rapid response in hippocampal tissue as well as in the CSF, represented by an increase in the activity of endogenous antioxidant enzymes SOD and CAT.
在对照组以及海马体和脑脊液中，分别于缺血5、10和15分钟后的5小时，以及缺血10分钟后的1天或2天测量超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。缺血后5小时总SOD活性显著增加，主要是由于铜锌超氧化物歧化酶（CuZn-SOD）活性增加所致。缺血5分钟后5小时测得的值最高（增加160%），而使用缺血15分钟时测得的值最小（增加40%）。与海马体相比，脑脊液中SOD的活性在所有缺血间隔后均同等增加。海马体缺血10分钟后，总SOD和CuZn-SOD的活性仅在再灌注5小时和24小时后显著增加，但在脑脊液中，它们在所有检查的再灌注间隔后均增加。缺血5分钟（增加260%）、10分钟和15分钟（增加100%）后，海马体中CAT的活性显著增加。脑脊液中CAT的活性在所有缺血间隔后均同等增加（增加200%）。缺血发作会导致海马组织以及脑脊液中出现快速反应，表现为内源性抗氧化酶SOD和CAT的活性增加。
